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Detection of early ischemic myocardial damage using immunohistochemical technique with anti-actin antibody in humans and rats
https://kindai.repo.nii.ac.jp/records/2003735
https://kindai.repo.nii.ac.jp/records/2003735871867a4-a86e-4ea5-86c9-f74e217695a3
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AA0050842X-19871200-0207.pdf (2.0 MB)
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| アイテムタイプ | 紀要論文 / departmental bulletin paper(1) | |||||||
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| 公開日 | 2025-09-22 | |||||||
| タイトル | ||||||||
| タイトル | Detection of early ischemic myocardial damage using immunohistochemical technique with anti-actin antibody in humans and rats | |||||||
| 言語 | en | |||||||
| 作成者 |
Nishida, Shozo
× Nishida, Shozo
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| 言語 | ||||||||
| 言語 | eng | |||||||
| キーワード | ||||||||
| 主題 | actin, myocardial ischemia, myocardial infarction, immunohistochemistry | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | The effectiveness of diagnosing myocardial infarction, and changes of actin filaments were investigated in the human autopsy cases and the experimental ischemia of rats, by using immunohistochemical methods with anti-actin antibodies produced from chicken gizzard. In acute myocardial infarction of human materials, the diminished areas of immunohistochemical reactions with anti-actin antibodies were slightly larger than the areas losing the triphenyltetrazolium chloride (TTC) reaction. In the experimental study, the left coronary arteries of rats were ligated to produce ischemia. In the cases of ischemia lasting for 5, 15 and 30 minutes, TTC reaction was shown, but the anti-actin antibody reaction disappeared in the areas corresponding to the ischemic sites. While actin filaments were clearly revealed on electron microscopic (EM) examination of these sites. In the case of ischemia lasting for more than 6 hours, the anti-actin antibody reaction had disappeared, corresponding to the disappearance of the TTC reaction. At this stage, actin filaments were revealed by EM examination, These results indicate that ischemia lasting more than 5 minutes induces some type of biochemical degeneration at the molecular level of myocardial actin, most likely the change of actin polymerization. Moreover, they show that the anti-actin antibody technique is capable of detecting such very early degenerative and ischemic changes, proving itself to be better suited for early determination of the range and degree of ischemia in humans and rats. | |||||||
| 言語 | en | |||||||
| 出版者 | ||||||||
| 出版者 | The Kinki University Medical Association | |||||||
| 言語 | en | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | departmental bulletin paper | |||||||
| 出版タイプ | ||||||||
| 出版タイプ | AM | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||
| 収録物識別子 | ||||||||
| 収録物識別子タイプ | PISSN | |||||||
| 収録物識別子 | 03866092 | |||||||
| 開始ページ | ||||||||
| 開始ページ | 207 | |||||||
| 終了ページ | ||||||||
| 終了ページ | 220 | |||||||
| 書誌情報 |
en : ACTA MEDICA KINKI UNIVERSITY 巻 12, 号 2, p. 207-220, 発行日 1987-12 |
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