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A novel keratohyalin granules protein : the 55 kd acidic protein
https://kindai.repo.nii.ac.jp/records/2003716
https://kindai.repo.nii.ac.jp/records/2003716fdd4c1c0-13c2-4962-982a-4beab25782c1
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AA0050842X-19881200-0139.pdf (696.8 KB)
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| アイテムタイプ | 紀要論文 / departmental bulletin paper(1) | |||||||||
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| 公開日 | 2025-09-22 | |||||||||
| タイトル | ||||||||||
| タイトル | A novel keratohyalin granules protein : the 55 kd acidic protein | |||||||||
| 言語 | en | |||||||||
| 作成者 |
Tezuka, Tadashi
× Tezuka, Tadashi
× Takahashi, Masae
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| 言語 | ||||||||||
| 言語 | eng | |||||||||
| キーワード | ||||||||||
| 主題 | keratinocyte, differentiation marker, membrane lining protein, keratohyalin granules, the stratum corneum | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | A monoclonal antibody (Ted-R-1) was developed by immunizing mice with pI 4.7, a hematoxylin stainable fraction of the extract in a 50 mM Tris-HCl buffer, pH 7.3, containing 10μg/ml phenylmethylsulfonyl fluoride from the newborn rat whole epidermis. An indirect immunofluorescent technique revealed that Ted-R-1 was located in two places: 1) keratohyalin granules and 2) the cell membrane region of the stratum corneum. Further the immunoblotting analysis of pI 4.7, the antigenic fraction which was used to produce Ted-R-1, demonstrated that there was only a single 55 kd polypeptide with which Ted-R-1 could react. No positive spot was seen in the keratin proteins. The major amino acids of the 55 kd, pI 4.7 protein, were glycine (24.4%), serine (17.3%), glutamic acid (14.1%) and alanine (7.7%), but the histidine residue was only 3.1%. Judging from the amino acid composition, this protein is considered to be the third component of the keratohyalin granules. | |||||||||
| 言語 | en | |||||||||
| 出版者 | ||||||||||
| 出版者 | The Kinki University Medical Association | |||||||||
| 言語 | en | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | departmental bulletin paper | |||||||||
| 出版タイプ | ||||||||||
| 出版タイプ | AM | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||
| 収録物識別子 | ||||||||||
| 収録物識別子タイプ | PISSN | |||||||||
| 収録物識別子 | 03866092 | |||||||||
| 開始ページ | ||||||||||
| 開始ページ | 139 | |||||||||
| 終了ページ | ||||||||||
| 終了ページ | 145 | |||||||||
| 書誌情報 |
en : ACTA MEDICA KINKI UNIVERSITY 巻 13, 号 2, p. 139-145, 発行日 1988-12 |
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