| アイテムタイプ |
紀要論文 / departmental bulletin paper(1) |
| 公開日 |
2025-09-22 |
| タイトル |
|
|
タイトル |
Combination of exonucleolytic and polymerase activities in a purified rat liver enzyme |
|
言語 |
en |
| 作成者 |
Tomura, T. Takanori
Sueyoshi, Akihiro
Van Lancker, L. Julien
|
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
mammalian DNA polymerase, exonucleolytic activity, DNA repair, rat liver |
| 内容記述 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
In bacteria, in vitro repair of thymine dimers can be achieved by the sequential action of a Micrococcus luteus endonuclease and DNA polymerase I. The repair activity of DNA polymerase I is associated with its 5'→3' exonucleolytic activity. DNA polymerase activities were purified from rat liver. Partially purified enzyme was passed through a DNA cellulose column. The DNA has previously been nicked with a repair endonuclease purified from rat liver. Three fractions were obtained. The fractions were then eluted on a SDS Sephadex G200 column. The first fraction (170,000 daltons) has only DNA polymerase activity, the second (40,000 daltons) has both 5'→3' exonuclease and DNA polymerase activity. The third fraction (18,000 to 20,000 daltons) has only exonucleolytic activity. The second fraction was recovered with both polymerase and exonuclease activities. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
The Kinki University Medical Association |
|
言語 |
en |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 収録物識別子 |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
03866092 |
| 開始ページ |
|
|
開始ページ |
299 |
| 終了ページ |
|
|
終了ページ |
305 |
| 書誌情報 |
en : ACTA MEDICA KINKI UNIVERSITY
巻 14,
号 2,
p. 299-305,
発行日 1989-12
|
AA0050842X-19891200-0299.pdf (450.5 KB)
