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Endocrine therapy sensitivity test for breast cancer using collagen gel droplet embedded culture
https://kindai.repo.nii.ac.jp/records/2003151
https://kindai.repo.nii.ac.jp/records/2003151892b67f1-c37c-4eb0-b8d9-bf33d912c70e
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AA0050842X-19950900-0197.pdf (1.0 MB)
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| Item type | 紀要論文 / departmental bulletin paper(1) | |||||||||||
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| 公開日 | 2025-07-09 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Endocrine therapy sensitivity test for breast cancer using collagen gel droplet embedded culture | |||||||||||
| 言語 | en | |||||||||||
| 作成者 |
Nishiyama, Kouichiro
× Nishiyama, Kouichiro
× Hara, Satoshi
× Wada, Tomio
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| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| キーワード | ||||||||||||
| 主題 | Drug sensitivity test, Endocrine therapy, breast cancer, In vitro endocrine therapy sensitivity test, Collagen gel droplet embedded culture drug sensitivity test (CD-DST) | |||||||||||
| 内容記述 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | To establish a new in vitro endocrine therapy sensitivity test that enables use to easily and accurately predict the efficacy of endocrine therapeutic agents after breast cancer surgery, we assessed the usefulness of a collagen gel droplet embedded culture drug sensitivity test (CD-DST), a chemosensitivity test using collagen droplet culture. Using the endocrine therapeutic agents tamoxifen (TAM) and medroxyprogesterone acetate (MPA), the optimal conditions of drug exposure and the cut-off point of efficacy evaluation were determined. TAM was also used to determine the sensitivity of clinical materials by this method. Four human breast cancer cell lines [Estrogen receptor (ER)- and Progesterone receptor (PgR)-positive MCF-7 and ZR-75-1,and ER- and PgR-negative MRK-nu-1 and SK-Br-3] and clinical materials from 22 patients were examined in the tests. After exposure to TAM at 10⁻⁷ M, and MPA at 10⁻⁵ M, the Treated/Contorl (T/C)(%) for the receptor-positive cell line was significantly lower than that for the receptor-negative cell line (p<0.01). The conditions of TAM, and MPA exposure were set as continuous exposure at 10⁻⁷ M, 10⁻⁵ M, and the drug was considered "effective" at a T/C (%)≤60%. TAM, and MPA was evaluated as "effective" against the receptor-positive cells and "ineffective" against the receptor-negative cells. In patients who underwent postoperative adjuvant therapy with TAM, who were tested resistant to TAM by the CD-DST method tended to develop recurrence. These findings suggest that CD-DST can select appropriate endocrine therapeutic agents for breast cancer and predict their anticancer effects under optimal conditions of tumor exposure to TAM and MPA, and the cut-off point for efficiency evaluation. | |||||||||||
| 言語 | en | |||||||||||
| 出版者 | ||||||||||||
| 出版者 | The Kinki University Medical Association | |||||||||||
| 言語 | en | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||
| 出版タイプ | ||||||||||||
| 出版タイプ | AM | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||
| 収録物識別子 | ||||||||||||
| 収録物識別子タイプ | PISSN | |||||||||||
| 収録物識別子 | 03866092 | |||||||||||
| 開始ページ | ||||||||||||
| 開始ページ | 197 | |||||||||||
| 終了ページ | ||||||||||||
| 終了ページ | 209 | |||||||||||
| 書誌情報 |
en : ACTA MEDICA KINKI UNIVERSITY 巻 20, 号 3, p. 197-209, 発行日 1995-09 |
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