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Pathophysiological characteristics of mitochondria and structural abnormality of mitochondrial DNA in hypertrophied myocardium of stroke-prone SHR
https://kindai.repo.nii.ac.jp/records/2003148
https://kindai.repo.nii.ac.jp/records/20031489f9fe5a7-6ae2-47e8-94c3-d8e8250105b1
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AA0050842X-19950900-0169.pdf (2.1 MB)
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| Item type | 紀要論文 / departmental bulletin paper(1) | |||||||
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| 公開日 | 2025-07-09 | |||||||
| タイトル | ||||||||
| タイトル | Pathophysiological characteristics of mitochondria and structural abnormality of mitochondrial DNA in hypertrophied myocardium of stroke-prone SHR | |||||||
| 言語 | en | |||||||
| 作成者 |
Tokoro, Tadao
× Tokoro, Tadao
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| 言語 | ||||||||
| 言語 | eng | |||||||
| キーワード | ||||||||
| 主題 | cardiac hypertrophy, mitochondria, DNA, SHRSP, WKY, free radical(s), superoxide dismutase | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | To clarify the importance of mitochondrial alteration for pathogenesis in stroke-prone SHR (SHRSP) hypertrophied heart, morphological and biochemical changes in isolated mitochondria were examined at prehypertensive and hypertensive stages, in comparison with those in Wistar-Kyoto rats (WKY). Furthermore, the structural alteration in mitochondrial DNA (mtDNA) was examined by restriction fragment length polymorphism (RFLP) analysis. At 5 weeks of age (prehypertensive stage), although the blood pressure of SHRSP was already higher than that of WKY, no significant differences were found in any parameters examined in the present study. At 24 weeks of age (hypertensive stage), SHRSP showed marked cardiomegaly, mainly due to left ventricular hypertrophy. In such hypertrophic myocardium, focal degeneration of myocardial cells and interstitial fibrosis were observed throughout the left ventricle. Isocitrate dehydrogenase (ICDH), which is related to energy production, was significantly lower in 24-week-old SHRSP myocardium than in WKY. Furthermore, superoxide dismutase (SOD), a potent radical scavenger, was also lower in 24-week-old SHRSP myocardium. On the other hand, RFLP analysis by Rsa I revealed the two deletions in the electrophoresis of fragments in the 24-week-old SHRSP myocardium, but not in the liver. These findings clearly indicate the decrease of mitochondrial function in hypertensive stage showing severe left ventricular hypertrophy. Although the precise mechanisms of such dysfunction were unclear, some of them may be related, at least partly, to mtDNA alteration. Free radical(s) may be a key factor for such mitochondrial changes, because the mitochondria is the major source of free radical generation and SOD activity may be inadequate for elimination of free radical(s) in SHRSP heart. | |||||||
| 言語 | en | |||||||
| 出版者 | ||||||||
| 出版者 | The Kinki University Medical Association | |||||||
| 言語 | en | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | departmental bulletin paper | |||||||
| 出版タイプ | ||||||||
| 出版タイプ | AM | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||
| 収録物識別子 | ||||||||
| 収録物識別子タイプ | PISSN | |||||||
| 収録物識別子 | 03866092 | |||||||
| 開始ページ | ||||||||
| 開始ページ | 169 | |||||||
| 終了ページ | ||||||||
| 終了ページ | 179 | |||||||
| 書誌情報 |
en : ACTA MEDICA KINKI UNIVERSITY 巻 20, 号 3, p. 169-179, 発行日 1995-09 |
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