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アイテム
Mechanism of platelet rich plasma clot lysis with anticoagulant and antiplatelet agents
https://kindai.repo.nii.ac.jp/records/2003137
https://kindai.repo.nii.ac.jp/records/2003137ba395a5c-1979-4881-8354-85b55b290840
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AA0050842X-19951200-0331.pdf (1.3 MB)
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| アイテムタイプ | 紀要論文 / departmental bulletin paper(1) | |||||||
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| 公開日 | 2025-07-09 | |||||||
| タイトル | ||||||||
| タイトル | Mechanism of platelet rich plasma clot lysis with anticoagulant and antiplatelet agents | |||||||
| 言語 | en | |||||||
| 作成者 |
Yuasa, Haruyuki
× Yuasa, Haruyuki
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| 言語 | ||||||||
| 言語 | eng | |||||||
| キーワード | ||||||||
| 主題 | dextran sulfate, (E)-3-[p-(1H-imidazol-1-ylmethyl) phenyl]-2-propenoic acid, platelet rich plasma clot lysis | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Resistance of a platelets-rich thrombus to lysis limits the thrombolytic therapy by plasminogen activators. To clarify the influence of dextran sulfate and (E)-3- [p-(1H-imidazol-1-ylmethyl) phenyl]-2-propenoic acid upon thrombolysis, effects of these substances on clot lysis of platelet rich plasma (PRP) by urokinase-type plasminogen activator (u-PA) were studied. The extent of clot lysis was assessed as the time (t1/2) when the turbidity of clot reached to the half of the maximum. The t1/2 value was significantly shortened by dextran sulfate or (E)-3-[p-(1H-imidazol-1-ylmethyl) phenyl]-2-propenoic acid in a dose-dependent. Also, coexistence of dextran sulfate and (E)-3-[p-(1H-imidazol-1-ylmethyl) phenyl] -2-propenoic acid enhanced PRP clot lysis. Electrophoretic enzymography was showed that dextran sulfate enhanced u-PA activity in the purified system (118-121% of control), the one in euglobulin fraction (124-127% of control), and the one in euglobulin fraction with exogenous u-PA (148-151% of control). However, (E)-3-[p-(1H-imidazol-1-ylmethyl) phenyl]-2-propenoic acid did not affect u-PA activity (97-103% of control). The kinetic analysis of u-PA activity with S-2444 revealed that (E)-3-[p-(1H-imidazol-1-ylmethyl) phenyl] -2-propenoic acid did not affect u-PA activity. The electrophoretic enzymography and kinetic analysis suggested that dextran sulfate enhanced u-PA activity without contributing factor XII-prekallikrein system. The solubility of PRP clot by urea was decreased by (E)-3-[p-(1H-imidazol-1-ylmethyl) phenyl]-2-propenoic acid in a dose dependent. To evaluate density of clot, the value of maximal O.D. (max O.D.) during clot formation. The value of max O.D. was decreased by (E)-3-[p-(1H-imidazol-1-ylmethyl) phenyl]-2- propenoic acid in a dose-dependent, but was not by dextran sulfate. These results suggest that both dextran sulfate and (E)-3-[p-(1H-imidazol-1-ylmethyl) phenyl]-2-propenoic acid enhance PRP clot lysis additivity. | |||||||
| 言語 | en | |||||||
| 出版者 | ||||||||
| 出版者 | The Kinki University Medical Association | |||||||
| 言語 | en | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | departmental bulletin paper | |||||||
| 出版タイプ | ||||||||
| 出版タイプ | AM | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||
| 収録物識別子 | ||||||||
| 収録物識別子タイプ | PISSN | |||||||
| 収録物識別子 | 03866092 | |||||||
| 開始ページ | ||||||||
| 開始ページ | 331 | |||||||
| 終了ページ | ||||||||
| 終了ページ | 344 | |||||||
| 書誌情報 |
en : ACTA MEDICA KINKI UNIVERSITY 巻 20, 号 4, p. 331-344, 発行日 1995-12 |
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