{"created":"2025-07-10T00:26:38.068158+00:00","id":2003134,"links":{},"metadata":{"_buckets":{"deposit":"af41a995-3887-466a-bcaf-e1a13aecd6aa"},"_deposit":{"created_by":114,"id":"2003134","owners":[114],"pid":{"revision_id":0,"type":"depid","value":"2003134"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:02003134","sets":["14:923:1519:1751870583683"]},"author_link":[],"control_number":"2003134","item_30002_bibliographic_information29":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1995-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"310","bibliographicPageStart":"299","bibliographicVolumeNumber":"20","bibliographic_titles":[{"bibliographic_title":"ACTA MEDICA KINKI UNIVERSITY","bibliographic_titleLang":"en"}]}]},"item_30002_creator2":{"attribute_name":"作成者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Kinki University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Azuma, Teruyuki","creatorNameLang":"en"}]}]},"item_30002_description9":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Chronic myelogenous leukemia is characterized by the Philadelphia chromosome, which results from the fusion of the bcr gene and the abl gene. The bcr-abl fusion gene encodes a tyrosine kinase with transforming activity. Inhibition of bcr-abl gene expression may have profound effects on the cell biology of Ph positive cells and become a therapeutic technique. In this study, a synthesized ribozyme targeted to the b3a2 fusion gene was used. The ribozyme cleaved bcr-abl mRNA in cell free in vitro system. Then, this ribozyme was introduced into a murine myeloproliferative sarcome virusbased retroviral vector. The recombinant retrovirus was infected to CML cell line, K562. The ribozyme decreased levels of bcr-abl mRNA in these cells, and inhibited cell growth partially. These results suggest that the ribozyme could be an effective therapeutic agent for the treatment of Ph positive leukemias.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_30002_file35":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"AA0050842X-19951200-0299.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://kindai.repo.nii.ac.jp/record/2003134/files/AA0050842X-19951200-0299.pdf"},"version_id":"a6e8ec47-1da6-4eb9-b908-07e7f04579aa"}]},"item_30002_language12":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_30002_page_end28":{"attribute_name":"終了ページ","attribute_value_mlt":[{"subitem_end_page":"310"}]},"item_30002_page_start27":{"attribute_name":"開始ページ","attribute_value_mlt":[{"subitem_start_page":"299"}]},"item_30002_publisher10":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"The Kinki University Medical Association","subitem_publisher_language":"en"}]},"item_30002_resource_type13":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_30002_source_identifier22":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"03866092","subitem_source_identifier_type":"PISSN"}]},"item_30002_subject8":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ribozyme","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cleavage reaction","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"bcrabl","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"retroviral transduction","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"quantitative RT-PCR","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_30002_title0":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Ribozyme-mediated cleavage of the bcr-abl transcript","subitem_title_language":"en"}]},"item_30002_version_type15":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_title":"Ribozyme-mediated cleavage of the bcr-abl transcript","item_type_id":"40004","owner":"114","path":["1751870583683"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2025-07-09"},"publish_date":"2025-07-09","publish_status":"0","recid":"2003134","relation_version_is_last":true,"title":["Ribozyme-mediated cleavage of the bcr-abl transcript"],"weko_creator_id":"114","weko_shared_id":-1},"updated":"2025-10-15T06:39:58.552071+00:00"}