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An automated high-performance liquid chromatographic method for quantitating arbekacin and vancomycin in serum using coupled column chromatography
https://kindai.repo.nii.ac.jp/records/2003125
https://kindai.repo.nii.ac.jp/records/2003125e7ed1d7d-e4d1-485f-a137-ed6f81a00f22
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AA0050842X-19950600-0091.pdf (349.3 KB)
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| アイテムタイプ | 紀要論文 / departmental bulletin paper(1) | |||||||||
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| 公開日 | 2025-07-08 | |||||||||
| タイトル | ||||||||||
| タイトル | An automated high-performance liquid chromatographic method for quantitating arbekacin and vancomycin in serum using coupled column chromatography | |||||||||
| 言語 | en | |||||||||
| 作成者 |
Kitahashi, Toshihiro
× Kitahashi, Toshihiro
× Ohba, Yasuhiro
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| 言語 | ||||||||||
| 言語 | eng | |||||||||
| キーワード | ||||||||||
| 主題 | arbekacin, vancomycin, anti-MRSA drug, serum level, coupled column chromatography | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Arbekacin (ABK) and vancomycin (VCM) are used for treating infections caused by methicillin-resistant Staphylococcus aureus. A rapid, accurate, coupled column chromatographic procedure employing direct injection of microserum has been developed for ABK and VCM quantitation in human serum. The columns consist of a separation column filled with reversed ODS-C18 and a pretreatment column filled with macroporous hydrophilic copolymer. The procedure for ABK determination involves the counter-ion method with sodium octanesulfonate, subsequent derivatization with o-phthalaldehyde and fluorescence detection. A mixture of potassium phosphate buffer and acetonitrile is used as the mobile phase, and VCM is directly detected at a UV wavelength. Coefficients of variation for within-day reproducibility were 0-3.00% (x̄=1.2-21.1 μg/ml) and 1.08-2.88% (x̄=2.6-52.4 μg/ml) for quantitating ABK and VCM, respectively ; for between-day reproducibility they were 4.68% (x̄=2.5 μg/ml) and 3.47% (x̄=9.5 μg/ml), respectively, with recovery rates of 92-106% and 98-112%, respectively. The minimum limit of detection (S/N=3) was 0.02 μg/ml for the ABK assay and 0.05 μg/ml for the VCM assay. The correlation coefficients between the fluorescence polarization immunoassay and this method were 0.978 and 0.991 for ABK and VCM, respectively. No interference was found either from endogenous substances or from many currently used antibiotics, indicating good selectivity and high sensitivity. | |||||||||
| 言語 | en | |||||||||
| 出版者 | ||||||||||
| 出版者 | The Kinki University Medical Association | |||||||||
| 言語 | en | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | departmental bulletin paper | |||||||||
| 出版タイプ | ||||||||||
| 出版タイプ | AM | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||
| 収録物識別子 | ||||||||||
| 収録物識別子タイプ | PISSN | |||||||||
| 収録物識別子 | 03866092 | |||||||||
| 開始ページ | ||||||||||
| 開始ページ | 91 | |||||||||
| 終了ページ | ||||||||||
| 終了ページ | 95 | |||||||||
| 書誌情報 |
en : ACTA MEDICA KINKI UNIVERSITY 巻 20, 号 2, p. 91-95, 発行日 1995-06 |
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