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Genetic alterations in sporadic colorectal carcinomas at all clinical stages of progression
https://kindai.repo.nii.ac.jp/records/2003109
https://kindai.repo.nii.ac.jp/records/20031091264f99a-26f2-4d3a-b050-de7e07d44469
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AA0050842X-19950300-0013.pdf (1.4 MB)
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| Item type | 紀要論文 / departmental bulletin paper(1) | |||||||||
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| 公開日 | 2025-07-08 | |||||||||
| タイトル | ||||||||||
| タイトル | Genetic alterations in sporadic colorectal carcinomas at all clinical stages of progression | |||||||||
| 言語 | en | |||||||||
| 作成者 |
Inui, Hiroki
× Inui, Hiroki
× Watatani, Masahiro
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| 言語 | ||||||||||
| 言語 | eng | |||||||||
| キーワード | ||||||||||
| 主題 | colorectal cancer, APC, p53, K-ras, point mutation, loss of heterozygosity | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | The accumulation of genetic alterations during the adenoma-carcinoma sequence is associated with the development and/or progression of colorectal carcinoma. To examine whether alterations in APC, K-ras, and p53 genes, and allelic loss of 18q play a role in the late stage of progression of colorectal carcinoma, DNA samples from 40 patients with colorectal carcinoma at all clinical stages (7 Dukes A, 14 Dukes B, 19 Dukes C) were studied for loss of heterozygosity (LOH) at the loci on chromosomes 5q21, 17p13 and 18q21, using restriction fragment length polymorphism analysis. In addition, mutations in the APC, K-ras and p53 genes were examined by RNase protection analysis and subsequent sequencing analysis. LOH at 5q21, 17p13 and 18q21 was identified in 31%, 74%, and 59% of the informative tumor samples, respectively. Somatic mutations in APC, K-ras, and p53 gene were detected in 38%, 38%, and 53% of the 40 carcinomas, respectively. There was a significant correlation between genetic alterations in the APC and K-ras genes (p<0.01). With progressive Dukes' stages, the frequency of genetic alterations increased. Five of 19 Dukes C tumors showed more than three genetic alterations. However, only one Dukes A tumor had three alterations. These findings suggest that tumor cells with an increased number of genetic alterations may contribute to malignant progression in colorectal carcinoma. | |||||||||
| 言語 | en | |||||||||
| 出版者 | ||||||||||
| 出版者 | The Kinki University Medical Association | |||||||||
| 言語 | en | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | departmental bulletin paper | |||||||||
| 出版タイプ | ||||||||||
| 出版タイプ | AM | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||
| 収録物識別子 | ||||||||||
| 収録物識別子タイプ | PISSN | |||||||||
| 収録物識別子 | 03866092 | |||||||||
| 開始ページ | ||||||||||
| 開始ページ | 13 | |||||||||
| 終了ページ | ||||||||||
| 終了ページ | 21 | |||||||||
| 書誌情報 |
en : ACTA MEDICA KINKI UNIVERSITY 巻 20, 号 1, p. 13-21, 発行日 1995-03 |
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