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Enhanced detection of active oxygen by chemiluminescence
https://kindai.repo.nii.ac.jp/records/2002976
https://kindai.repo.nii.ac.jp/records/200297669f86a1b-db85-4712-9017-ee4b3d3d75b7
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AA0050842X-19981200-0079.pdf (337.2 KB)
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| Item type | 紀要論文 / departmental bulletin paper(1) | |||||||||||
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| 公開日 | 2025-06-20 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Enhanced detection of active oxygen by chemiluminescence | |||||||||||
| 言語 | en | |||||||||||
| 作成者 |
Nozaki, Osamu
× Nozaki, Osamu
× Iwaeda, Toshinao
× Kato, Yoshio
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| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| キーワード | ||||||||||||
| 主題 | active oxygen, chemiluminescence, enhancement, imidazole, luminol/HRP, POCL | |||||||||||
| 内容記述 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | We investigated factors that enhance chemiluminescence (CL) detection of active oxygen generated by imidazole catalysis from dopamine using a flow injection system and peroxyoxalate chemiluminescence. The factors investigated were the sources of imidazole, the effect of perchlorate, and the types of chemiluminescence detection systems (luminol/peroxidase, peroxyoxalate chemiluminescence (TDPO/perylene), luminol/ferricyanide, lucigenin, pyrogallol, purpurogallin, luminol). Rank order of light intensity for the imidazole sources was Katayama (100%)>Kishida (72)>Wako (52.7)>Kanto (50.8)>Nakarai (43.5). Perchlorate (0,10,25,50 and 100mmol/L) incubated with the imidazole solution containing dopamine decreased the background light, and perchlorate (50 and 100mmol/L) elevated the signal to background ratio 16.4% and 26.6%, respectively. Rank order for the CL detection systems was luminol/peroxidase (31,700 RLU)>peroxyoxalate chemiluminescence (854)>luminol/ferricyanide (530)>lucigenin (128)>pyrogallol (6.6)>purpurogallin (3.1)>luminol (2.6). The imidazole from Katayama, the addition of perchlorate, and the detection by luminol/horseradish peroxidase chemiluminescence were effective for enhanced CL detection of active oxygen. | |||||||||||
| 言語 | en | |||||||||||
| 出版者 | ||||||||||||
| 出版者 | The Kinki University Medical Association | |||||||||||
| 言語 | en | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||
| 出版タイプ | ||||||||||||
| 出版タイプ | AM | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||
| 収録物識別子 | ||||||||||||
| 収録物識別子タイプ | PISSN | |||||||||||
| 収録物識別子 | 03866092 | |||||||||||
| 開始ページ | ||||||||||||
| 開始ページ | 79 | |||||||||||
| 終了ページ | ||||||||||||
| 終了ページ | 83 | |||||||||||
| 書誌情報 |
en : ACTA MEDICA KINKI UNIVERSITY 巻 23, 号 3/4, p. 79-83, 発行日 1998-12 |
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