WEKO3
アイテム
Epidemiological and molecular biological studies of azole resistance in Candida albicans
https://kindai.repo.nii.ac.jp/records/2002975
https://kindai.repo.nii.ac.jp/records/2002975009951f3-97b6-477d-8984-10604580626a
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AA0050842X-19981200-0065.pdf (1.2 MB)
|
|
| アイテムタイプ | 紀要論文 / departmental bulletin paper(1) | |||||||
|---|---|---|---|---|---|---|---|---|
| 公開日 | 2025-06-20 | |||||||
| タイトル | ||||||||
| タイトル | Epidemiological and molecular biological studies of azole resistance in Candida albicans | |||||||
| 言語 | en | |||||||
| 作成者 |
Yamazumi, Toshiaki
× Yamazumi, Toshiaki
|
|||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| キーワード | ||||||||
| 主題 | Candida albicans, antifungal susceptibility, azole resistance, BENr gene, induced resistance | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Resistance to antifungal agents, especially azole agents such as fluconazole, in the opportunistic yeast Candida albicans is increasing in the U.S.A. and Europe. We studied the present state of antifungal resistance in our hospital and the development and potential mechanisms of resistance. The antifungal susceptibility of 285 C. albicans strains isolated over the last two years was investigated using the broth microdilution method. Most of the isolates showed relatively low MIC values in all five agents studied. However, two resistant strains to flucytosine and one resistant strain to fluconazole were found. The latter showed cross resistance to miconazole and itraconazole. In vitro induction of resistance with fluconazole was attempted. The four fluconazol susceptible strains were passaged in liquid yeast nitrogen base medium containing fluconazole. Fluconazole resistance developed in two out of four strains after serial passages. The length of time required for resistant induction differed between the two strains. Moreover, quantitative analysis of mRNA using reverse transcription polymerase chain reaction revealed increased mRNA levels of BENr gene, which codes drug efflux pump, in induced resistant strains derived from 95-77. The mRNA levels of BENr gene in 96-579,in which trailing phenomenon was clearly seen in the susceptibility testing, were high even in parent strain. These findings indicate that emergence of azole resistance is related to continuous antifungal exposure, and that the ability for acquisition of resistance differs among strains. | |||||||
| 言語 | en | |||||||
| 出版者 | ||||||||
| 出版者 | The Kinki University Medical Association | |||||||
| 言語 | en | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | departmental bulletin paper | |||||||
| 出版タイプ | ||||||||
| 出版タイプ | AM | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||
| 収録物識別子 | ||||||||
| 収録物識別子タイプ | PISSN | |||||||
| 収録物識別子 | 03866092 | |||||||
| 開始ページ | ||||||||
| 開始ページ | 65 | |||||||
| 終了ページ | ||||||||
| 終了ページ | 77 | |||||||
| 書誌情報 |
en : ACTA MEDICA KINKI UNIVERSITY 巻 23, 号 3/4, p. 65-77, 発行日 1998-12 |
|||||||
