| アイテムタイプ |
紀要論文 / departmental bulletin paper(1) |
| 公開日 |
2025-06-20 |
| タイトル |
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|
タイトル |
SD-3212 blocks the inward rectifier potassium channel in guinea pig ventricular myocytes |
|
言語 |
en |
| 作成者 |
Akamatsu, Kan-Ichiro
Takai, Hiroyuki
Izawa, Hiroshi
Ishikawa, Kinji
|
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
inward rectifier potassium channel, SD-3212, ventricular myocytes |
| 内容記述 |
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|
内容記述タイプ |
Abstract |
|
内容記述 |
SD-3212 inhibited the calcium current, the sodium current and the muscarinic acetylcholine-receptor-operated potassium current. Inward rectifier potassium current (IK1)is considered to contribute to both the repolarization phase of the ventricular action potential and the maintenance of the resting potential. However, the kinetics of blocking effects of multiple channel blockers on the IK1 are unclear. In the present study, we investigated the effects of SD-3212 on the IK1 channel in isolated guinea pig ventricular myocytes. Male guinea pig heart was perfused by Langendorff's method to prepare isolated ventricular myocytes using collagenase. Membrane currents of the isolated ventricular myocytes were recorded using the patch clamp technique. The peak and steady-state IK1 were constantly inhibited by 100μM SD-3212 irrespective of the potentials applied (the steady-state current was 36±4% blocked and the peak current was 28±2% blocked) under whole cell clamp conditions. Slope conductance 35±3pS was unchanged. SD-3212 (1mM) decreased the open probability from 92.5±0.8% in the control to 90.2±l.8% after 2 min, 85.5±2.4% after 3 min, 64.7±3.2% after 4 min, and to 22.6±2.4% after 5 min of exposure. Mean open time and mean closed time were unchanged after 5 min of exposure. SD-3212 decreased the open probability from 92.5±0.8% under control conditions to 91.6±1.4% in the presence of 100μM SD-3212, 63.3±2.4% with 500μM SD-3212 and 22.6±3.1% with 1mM SD-3212 after 5 min of exposure under single channel recordings. In conclusions, SD-3212 blocks the IK1 channels in a high dose concentration-dependent manner. SD-3212 blocks IK1 channels via a hydrophobic pathway in accordance with a slow blocking mode. |
|
言語 |
en |
| 出版者 |
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|
出版者 |
The Kinki University Medical Association |
|
言語 |
en |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 収録物識別子 |
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収録物識別子タイプ |
PISSN |
|
収録物識別子 |
03866092 |
| 開始ページ |
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|
開始ページ |
1 |
| 終了ページ |
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|
終了ページ |
8 |
| 書誌情報 |
en : ACTA MEDICA KINKI UNIVERSITY
巻 23,
号 1/2,
p. 1-8,
発行日 1998-06
|
AA0050842X-19980600-0001.pdf (638.4 KB)
