| アイテムタイプ |
紀要論文 / departmental bulletin paper(1) |
| 公開日 |
2025-06-18 |
| タイトル |
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|
タイトル |
Mechanisms of retinoid resistance in a myeloid cell line, YM711,transfected with PML/RARα cDNA : possible involvement of redox potential |
|
言語 |
en |
| 作成者 |
Sumimoto, Yoshiyasu
Maeda, Yasuhiro
Nawata, Hiroyuki
Matsuda, Mitsuhiro
Kanamaru, Akihisa
|
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
transfection, YM711, retinoid resistance, PML/RARα, MDR-1, CRABP |
| 内容記述 |
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|
内容記述タイプ |
Abstract |
|
内容記述 |
We established a myeloid cell line (YM711) by transfecting exogenous PML/RARα cDNA into peripheral blood stem cells. The established cells were positive for CD33,CD34,CD38,CD13,CD14,and CD11b. Cytochemical examination also revealed YM711 cells to be positive for peroxidase, α-naphtyl butyrate esterase, and acid phosphatase. Although PML/ RARα mRNA was detected by reverse-transcription and polymerase chain reaction (RT-PCR) in YM711 cells, all-trans retinoic acid (ATRA) did not induce differentiation of these cells. We, therefore, designated the YM711 as being ATRA resistant. Because YM711 expressed multi-drug resistance 1 (MDR-1) mRNA and cell surface p-glycoprotein, we assessed whether verapamil and ATRA would induce the differentiation of YM711 and found not. Increased expression of cellular retinoic acid-binding protein-II (CRABP-II) was also detected on YM711 cells. We tried to suppress the expression of CRABP-II mRNA in vitro by using antisense-S-oligonucleotides for CRABP-II mRNA. However differentiation of YM711 was not observed in the presence of 10^<-5> M ATRA. To induce differentiation of this cell line, we then focused on redox regulation. We used thiol compounds-free RPMI 1640 medium, and mixed this with regular RPMI 1640 at the ratio of 3 : 7. After 4 days in culture with the 3 : 7 medium, the expression of CD14 on YM711 cells was enhanced significantly following the treatment with 10^<-6> M ATRA. In conclusion, we suggest that redox status is an important factor for ATRA-induced differentiation of the ATRA-resistant cell line. Furthermore, this cell line may be useful in elucidating the mechanism of resistance of leukemia cells to retinoids. |
|
言語 |
en |
| 出版者 |
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|
出版者 |
The Kinki University Medical Association |
|
言語 |
en |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 収録物識別子 |
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|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
03866092 |
| 開始ページ |
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|
開始ページ |
85 |
| 終了ページ |
|
|
終了ページ |
97 |
| 書誌情報 |
en : ACTA MEDICA KINKI UNIVERSITY
巻 24,
号 3/4,
p. 85-97,
発行日 1999-12
|
AA0050842X-19991200-0085.pdf (1.0 MB)
