| アイテムタイプ |
紀要論文 / departmental bulletin paper(1) |
| 公開日 |
2025-06-16 |
| タイトル |
|
|
タイトル |
ICAM-1 expression in a lymph node metastasis model of rat uterine corpus cancer in reference to metastatic activity |
|
言語 |
en |
| 作成者 |
Hashii, Koji
Tomura, T. Takanori
Tohya, Kazuo
Tateyama, Ichiro
|
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
Lymph node metastasis, Uterine corpus cancer, Walker 256 cells, ICAM-1, LFA-1 |
| 内容記述 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
To elucidate the mechanism of lymph node metastasis by tumor cells, we investigated rat Walker 256 (W256) cells with a low metastatic potential and a highly metastatic subline (PL3) that we established for sensitivity to immunocompetent cells involving ICAM-1/LFA-1-mediated cell adhesion. Immune electron microscopy revealed that cell adhesion mediated by ICAM-1/LFA-1 played a role in contact between W256 cells and lymphocytes. Immune electron microscopy also revealed that LFA-1 was expressed on the cells of marginal lymphatic sinues. These cells, forming lymphatic sinues, may be barriers to the infiltration of W256 cells with high ICAM-1 expression into the lymph nodes from sinues. Co-culture of PL3 cells with lymphocytes and co-culture of W256 cells with lymphocytes resulted in an increase in INF-γ and TNF-α in the culture solutions. The concentrations of these cytokines were found higher compared with cultures of lymph node cells alone. Flow cytometry showed that addition of IFN-γ and TNF-α increased the surface expression of ICAM-1 on W256 cells to a higher degree than on PL3 cells. In the lymph node microenvironment, cytokines may alter the surface expression of ICAM-1 on W256 and PL3 cells, and these changes may influence migration of tumor cells within the nodes and escape from immune surveillance. Such a mechanism may influence the formation of lymph node metastasis. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
The Kinki University Medical Association |
|
言語 |
en |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 収録物識別子 |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
03866092 |
| 開始ページ |
|
|
開始ページ |
77 |
| 終了ページ |
|
|
終了ページ |
82 |
| 書誌情報 |
en : ACTA MEDICA KINKI UNIVERSITY
巻 25,
号 3/4,
p. 77-82,
発行日 2000-12
|
AA0050842X-20001200-0077.pdf (776.6 KB)
