| アイテムタイプ |
紀要論文 / departmental bulletin paper(1) |
| 公開日 |
2025-06-12 |
| タイトル |
|
|
タイトル |
Effects of Nω-nitro-L-arginine-methyl-ester (L-NAME) on the production of leptin and tumor necrosis factor-α (TNF-α) in vivo and in vitro |
|
言語 |
en |
| 作成者 |
Iwai, Hiroshi
Ohno, Yasuhiro
Aoki, Norihiko
|
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
Adipocytes, Otsuka Long-Evans Tokushima Fatty rats, Nω-nitro-L-arginine-methyl-ester, nitric oxide, leptin, tumor necrosis factor-α |
| 内容記述 |
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|
内容記述タイプ |
Abstract |
|
内容記述 |
Background. Adipocytes produce the physiological effectors, adipocytokines, and also play an important role in nitric oxide (NO) production in diabetes mellitus. However, the role of NO in adipocytokine production is unclear. We examined whether NO had the effects on insulin resistance and the production of leptin and tumor necrosis factor-α (TNF-α). Methods. We administered Nω-nitro-L-arginine-methyl-ester (L-NAME, 10mg/100ml) solution, NO synthase inhibitor, to Otsuka Long-Evans Tokushima Fatty (OLETF) rats, a type 2 diabetic model, and to Long-Evans Tokushima Otsuka (LETO) rats, non-diabetic control. Blood levels of leptin and TNF-α were measured in the course of the experiment. We also investigated the effects of NO suppression on adipocytokines production in 3T3-L1 adipocytes, a murine adipocyte cell line. Results. L-NAME administration significantly elevated blood pressure at day 28 in the LETO rats and at day 14 and 28 in the OLETF rats, which suggested that L-NAME was effective for NO reduction. Blood levels of leptin and TNF-α in the L-NAME-treated rats were higher than those in no-treated rats. L-NAME induced insulin resistance at day 28 in the LETO rats and enhanced that in the OLETF rats. Leptin concentrations in the culture supernatant of 3T3-L1 adipocytes were increased by L-NAME in a dose-dependent manner (1-100μ mol/l). Conclusions. L-NAME enhanced production of adipocytokines in in vivo and in vitro experiment. These results provide a novel clue for elucidating the regulatory mechanism of adipocytokine production and the pathogenesis of type 2 diabetes mellitus. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
The Kinki University Medical Association |
|
言語 |
en |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 収録物識別子 |
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|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
03866092 |
| 開始ページ |
|
|
開始ページ |
27 |
| 終了ページ |
|
|
終了ページ |
34 |
| 書誌情報 |
en : ACTA MEDICA KINKI UNIVERSITY
巻 26,
号 2,
p. 27-34,
発行日 2001-12
|
AA0050842X-20011200-0027.pdf (684.0 KB)
