| アイテムタイプ |
紀要論文 / departmental bulletin paper(1) |
| 公開日 |
2025-05-20 |
| タイトル |
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|
タイトル |
The cellular origin of bone formed in a synthetic biodegradable polymer of polyglycolic acid fiber loaded with chicken osteoblasts |
|
言語 |
en |
| 作成者 |
Uemura, Fumiko
Isogai, Noritaka
Kamiishi, Hirosi
|
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
tissue engineering, osteoblast, biodegradable polymer |
| 内容記述 |
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|
内容記述タイプ |
Abstract |
|
内容記述 |
In the development of skeletal materials by tissue engineering, considerable questions remain as to the extent to which newly formed bone in osteoblast-polymer implants is derived from host or donor cells. To study the origin of bone cells that compose such implants, osteoblasts from calvariae of 16-day-old embryonic chicks were seeded onto a synthetic nonwoven polyglycolic acid (PGA) mesh. The cell-polymer constructs were then implanted into the subcutaneous space of immunologically non-reactive athymic nude mice. The constructs were recovered at 11, 18, and 28 days following implantation and prepared for histological and biochemical examinations. Nuclear staining of donor cells and immunoreactivity to an antibody specific for avian osteopontin, an extracellular matrix protein associated with mineralization were used to identify the origin of cells within the developing calcifying implant. Results from these studies suggest that the seeded donor cells were largely responsible for the observed mineralized tissue forming around the periphery of the constructs. These findings demonstrate the survival and localization of the transplanted osteoblasts which induce intramembranous ossification in these cell-polymer constructs. |
|
言語 |
en |
| 出版者 |
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|
出版者 |
The Kinki University Medical Association |
|
言語 |
en |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 収録物識別子 |
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収録物識別子タイプ |
PISSN |
|
収録物識別子 |
03866092 |
| 開始ページ |
|
|
開始ページ |
73 |
| 終了ページ |
|
|
終了ページ |
81 |
| 書誌情報 |
en : ACTA MEDICA KINKI UNIVERSITY
巻 27,
号 2,
p. 73-81,
発行日 2002-12
|
AA0050842X-20021200-0073.pdf (1.8 MB)
