| アイテムタイプ |
紀要論文 / departmental bulletin paper(1) |
| 公開日 |
2025-05-20 |
| タイトル |
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|
タイトル |
Regeneration of acutely injured renal tubules is promoted by a heparin-binding epidermal growth factor-like growth factor (HB-EGF) |
|
言語 |
en |
| 作成者 |
Wada, Norihisa
Takemura, Tsukasa
Okada, Mitsuru
Yoshioka, Kazuo
|
| 言語 |
|
|
言語 |
eng |
| キーワード |
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|
主題 |
soluble heparin-binding epidermal growth factor-like growth factor (HB-EGF), membrane-bound HB-EGF, acute tubular renal failure, juxtacrine activity |
| 内容記述 |
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|
内容記述タイプ |
Abstract |
|
内容記述 |
Background : Locally produced growth factors may mediate cell differentiation during regeneration following acute renal injury. Thus, we examined the participation of two different forms of heparin-binding epidermal growth factor (EGF)-like growth factor (HB-EGF), a new member of EGF family of growth factor, in regeneration of renal tubules after acute injury. Methods : To evaluate preventive and therapeutic effects of soluble (s) HB-EGF, recombinant (r) human (h) s-HB-EGF (250 μg/kg) was administered to BALB/c mice prior to or after onset of cisplatin-induced acute tubular renal failure (ATRF). Results : Administration after onset of ATRF suppressed increases in blood urea nitrogen (BUN) and serum creatinine (Cr). Pretreatment with rh s-HB-EGF showed a similar effect. Histologically evident tubular injuries, characterized by epithelial cell necrosis and detachment of cells from the tubular basement membrane were much milder in rh s-HB-EGF-treated mice than in untreated controls. Additionally, rh s-HB-EGF stimulated DNA synthesis in damaged renal epithelial cells according to 5-bromo-2'-deoxyuridine (BrdUrd) incorporation. To determine involvement of a precursor form of HB-EGF, proHB-EGF, in recovery from ATRF, we constructed a line of NRK 52E cells stably expressing proHB-EGF (NRKproHB-EGF) and cultured cells in three-dimensional type I collagen gels. NRKproHB-EGA formed tubule-like structures mimicking renal tubules, unlike NRK 52E cells transfected with empty vector alone (NRKvector), which formed only round colonies. Conclusions : HB-EGF may function importantly in resistance to and recovery from ATRF, and may have therapeutic potential. |
|
言語 |
en |
| 出版者 |
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|
出版者 |
The Kinki University Medical Association |
|
言語 |
en |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 収録物識別子 |
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収録物識別子タイプ |
PISSN |
|
収録物識別子 |
03866092 |
| 開始ページ |
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|
開始ページ |
9 |
| 終了ページ |
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|
終了ページ |
18 |
| 書誌情報 |
en : ACTA MEDICA KINKI UNIVERSITY
巻 27,
号 1,
p. 9-18,
発行日 2002-06
|
AA0050842X-20020600-0009.pdf (1000.9 KB)
