WEKO3
アイテム
Induction of odontoblasts from dental pulp cells followed by the formation of calcium and phosphate-rich nodules
https://kindai.repo.nii.ac.jp/records/2002839
https://kindai.repo.nii.ac.jp/records/2002839af2d2f8d-dfb6-43ff-b51a-29e9d0be2b82
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AA0050842X-20051200-0067.pdf (3.8 MB)
|
|
| アイテムタイプ | 紀要論文 / departmental bulletin paper(1) | |||||||
|---|---|---|---|---|---|---|---|---|
| 公開日 | 2025-05-14 | |||||||
| タイトル | ||||||||
| タイトル | Induction of odontoblasts from dental pulp cells followed by the formation of calcium and phosphate-rich nodules | |||||||
| 言語 | en | |||||||
| 作成者 |
Otaki, Sayuri
× Otaki, Sayuri
|
|||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| キーワード | ||||||||
| 主題 | calcification, odontoblast, dental pulp cell, cell culture, β-glycerophosphate | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Background: Tissue engineering for lost tissues is developing and that for teeth is no exception. Dental pulp cells have been successfully cultured and odontoblast induction by β-glycerophosphate have been reported. However, it has been unclear whether β-glycerophosphate acts on the differentiation of dental pulp cells into odontoblasts, or on the subsequent mineralizing process. Methods: I cultured dental pulp cells with and without β-glycerophosphate. The differentiation of dental pulp cells was analyzed by means of a Reverse Transcription Polymerase Chain Reaction (RT-PCR). The mineralization was analyzed by means of Electron probe microanalysis (EPMA). Results: In the absence or presence of glycerophosphate, earlier passage cells expressed collagen I and occasionally collagen III, markers of dental pulp cells. Also in the absence or presence of glycerophosphate, later passage cells expressed osteocalcin, a marker of odontoblasts in the secretory stage. In the presence of glycerophosphate, cells produced nodules which contained large amounts of calcium and phosphate. However, in the absence of glycerophosphate, no nodules were observed. Conclusions: Dental pulp cells have the potential to differentiate into odontoblasts without specific stimulation by β-glycerophosphate. β-glycerophosphate acts on the mineralizing process after differentiation. | |||||||
| 言語 | en | |||||||
| 出版者 | ||||||||
| 出版者 | The Kinki University Medical Association | |||||||
| 言語 | en | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | departmental bulletin paper | |||||||
| 出版タイプ | ||||||||
| 出版タイプ | AM | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||
| 収録物識別子 | ||||||||
| 収録物識別子タイプ | PISSN | |||||||
| 収録物識別子 | 03866092 | |||||||
| 開始ページ | ||||||||
| 開始ページ | 67 | |||||||
| 終了ページ | ||||||||
| 終了ページ | 75 | |||||||
| 書誌情報 |
en : ACTA MEDICA KINKI UNIVERSITY 巻 30, 号 2, p. 67-75, 発行日 2005-12 |
|||||||
