| アイテムタイプ |
紀要論文 / departmental bulletin paper(1) |
| 公開日 |
2025-05-09 |
| タイトル |
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|
タイトル |
Effects of apoptosis and estrogen on aging changes in female rat parotids |
|
言語 |
en |
| 作成者 |
Kusunoki, Takeshi
Shiraishi, Hiroshi
Murata, Kiyotaka
|
| 言語 |
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|
言語 |
eng |
| キーワード |
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主題 |
female rat parotid, aging, estrogen, apoptosis, ovariectomy, TUNEL staining |
| 内容記述 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Background : Generally, postclimacteric women show increased susceptiblity to xerostomia, and correlations between xerostomia and sex hormones have thus been the subject of study. Some researchers have examined the influence of sex hormones on the salivary glands of female rats following ovariectomy and recently, relations between aging symptoms and apoptosis have been noticed. In this study, we observed apoptosis during natural aging in female rat parotid by TUNEL staining, and also examined the influence estrogen on the apoptosis. Methods : Fifteen-week-old rat in the ovariectomy only groups underwent ovariectomy under intraperitoneal anesthesia by pentobarbital sodium. After confirming the absence of sexual cycle, the parotid glands were examined at 27 and 39 weeks. Another group received estrogen infusion following ovariectomy as above. Subsequently, a mini- osmotic pump containing estrogen dissolved in propylene glycol was subcutaneously implanted into the rat dorsum. TUNEL staining was performed for apoptosis detection by labeling the 3' OH ends of broken DNA using terminal deoxynucleotidyl transferase (TdT) using the In Apoptosis Detection Kit (Takara). Results : Our results showed that in natural aging groups, the TLI (TUNEL labeling index) mean of postmenopausal rats was significantly higher than in premenopausal rats. Also, in ovariectomized rats, TLI mean of rats with estrogen infusion after ovariectomy was significantly lower than in those receiving ovariectomy only. Conclusions : Our findings indicated that a reduction of estrogen could induce age related apoptotic changes in female rat parotid. |
|
言語 |
en |
| 出版者 |
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出版者 |
The Kinki University Medical Association |
|
言語 |
en |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 出版タイプ |
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|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 収録物識別子 |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
03866092 |
| 開始ページ |
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|
開始ページ |
27 |
| 終了ページ |
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|
終了ページ |
30 |
| 書誌情報 |
en : ACTA MEDICA KINKI UNIVERSITY
巻 29,
号 2,
p. 27-30,
発行日 2004-12
|
AA0050842X-20041200-0027.pdf (532.6 KB)
