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アイテム
3次元空間対応エレクトロポレーションシステムの開発に向けた初期検討
https://kindai.repo.nii.ac.jp/records/2002812
https://kindai.repo.nii.ac.jp/records/20028126b1afc16-e4ad-4798-8752-da773b841f6f
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AA12315376-20250315-0039.pdf (2.3 MB)
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| アイテムタイプ | 紀要論文 / departmental bulletin paper(1) | |||||||||
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| 公開日 | 2025-05-02 | |||||||||
| タイトル | ||||||||||
| タイトル | 3次元空間対応エレクトロポレーションシステムの開発に向けた初期検討 | |||||||||
| 言語 | ja | |||||||||
| タイトル | ||||||||||
| タイトル | Preliminary Study on a 3D Electroporation System | |||||||||
| 言語 | en | |||||||||
| 作成者 |
黄, 文敬
× 黄, 文敬
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| 言語 | ||||||||||
| 言語 | eng | |||||||||
| キーワード | ||||||||||
| 主題 | electroporation, cell transfection, electric field, 3D electrodes, tissue engineering | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Precise control of electric field distribution in electroporation systems is crucial for targeted gene transfection in three-dimensional (3D) space. However, conventional systems with two electrodes generate highly localized electric fields, leading to non-uniform transfection efficiency. This study proposes a novel electroporation system with a 3D electrode arrangement designed to achieve a broader and more controlled electric field distribution. The system employs a cuvette chamber with electrodes positioned on four sides of a square, and the electric field distribution was analyzed using the Finite Difference Method based on Laplace’s equation. Simulation results demonstrated that this electrode configuration significantly expands the effective electroporation area compared to conventional setups. A prototype cuvette chamber was fabricated, and electroporation experiments using FITC-dextran confirmed successful cell membrane poration. Despite the expanded electric field distribution in the three-quarter electrode condition, the number of FITC-positive cells remained comparable to the one-quarter condition, suggesting that additional factors influence transfection efficiency. These findings highlight the potential of the proposed electrode arrangement for improving electroporation uniformity, which may contribute to advancements in gene delivery and tissue engineering applications. | |||||||||
| 言語 | en | |||||||||
| 出版者 | ||||||||||
| 出版者 | 近畿大学工業高等専門学校 | |||||||||
| 言語 | ja | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | departmental bulletin paper | |||||||||
| 出版タイプ | ||||||||||
| 出版タイプ | AM | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||
| 収録物識別子 | ||||||||||
| 収録物識別子タイプ | PISSN | |||||||||
| 収録物識別子 | 18824374 | |||||||||
| 書誌情報 |
ja : 近畿大学工業高等専門学校研究紀要 en : Research reports Kindai University Technical College 号 18, p. 39-43, 発行日 2025-03-15 |
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