| アイテムタイプ |
紀要論文 / departmental bulletin paper(1) |
| 公開日 |
2025-05-08 |
| タイトル |
|
|
タイトル |
Detection of ABH blood group antigens in human cardiac muscle by the highly sensitive immunohistochemical staining method EnVision+ |
|
言語 |
en |
| 作成者 |
Yokota, Makoto
Sugiyama, Nobuko
Noda, Hiroshi
|
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
ABO blood grouping, EnVision+, cardiac muscle, putrefaction |
| 内容記述 |
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|
内容記述タイプ |
Abstract |
|
内容記述 |
We evaluated the detection of ABH antigens in paraformaldehyde-fixed human cardiac muscle by the EnVision+ system, which employs a dextran polymer reagent for the secondary antibody. Immunohistochemical microscopy of blood group A and B tissues using monoclonal anti-A and anti-B showed strong staining of the cardiac interstitium, but muscle fiber itself was negative. There was also extensive staining of the endothelial cells lining blood vessels. Cardiac muscle fibers were strongly stained by anti-H, but endothelial cells and smooth muscle of blood vessels were stained weakly and the interstitium was negative. Both endothelial cells lining blood vessels and the cardiac interstitium of blood group O tissues were strongly stained by anti-H, and cardiac muscle itself was stained weakly. In highly putrefactive cardiac muscle samples incubated at 37℃ in airtight tubes for one month, ABO blood group could be determined by the EnVision+ system. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
The Kinki University Medical Association |
|
言語 |
en |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 収録物識別子 |
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|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
03866092 |
| 開始ページ |
|
|
開始ページ |
11 |
| 終了ページ |
|
|
終了ページ |
17 |
| 書誌情報 |
en : ACTA MEDICA KINKI UNIVERSITY
巻 29,
号 1,
p. 11-17,
発行日 2004-06
|
AA0050842X-20040600-0011.pdf (2.1 MB)
