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Proteinase inhibitory activity of recombinant rabbit α-1-antiproteinases expressed in Escherichia coli
https://kindai.repo.nii.ac.jp/records/2002749
https://kindai.repo.nii.ac.jp/records/2002749ea2c36a0-af66-453f-a49b-037dae6b80e5
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AA0050842X-20071200-0057.pdf (937.1 KB)
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| アイテムタイプ | 紀要論文 / departmental bulletin paper(1) | |||||||
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| 公開日 | 2025-04-22 | |||||||
| タイトル | ||||||||
| タイトル | Proteinase inhibitory activity of recombinant rabbit α-1-antiproteinases expressed in Escherichia coli | |||||||
| 言語 | en | |||||||
| 作成者 |
Saito, Akio
× Saito, Akio
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| 言語 | ||||||||
| 言語 | eng | |||||||
| キーワード | ||||||||
| 主題 | α-1-antiproteinase, α-1-antitrypsin, serpin, recombinant proteins | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | The S-22 form of rabbit α-1-antiproteinase (α-1-antitrypsin), having tyrosine at the reactive site, was isolated from a rabbit liver cDNA library, sequenced and expressed in Escherichia coli. The recombinant fusion protein of the S-22 form with maltose binding protein did not inhibit elastase, trypsin, or chymotrypsin. However, the fusion proteins of the F isoform of rabbit α-1-antiproteinase and human α-1-antitrypsin inhibited those proteinases. The expression plasmid harboring cDNA for the S-22 and F forms were excised with BbsI and the resulting 0.5 kbase fragments were exchanged and ligated. The plasmids having a chimera sequence of the S-22 and F forms were expressed and the fusion proteins were examined for proteinase inhibitory activity. One of the fusion proteins, having 88 amino acids of the F form in the C-terminal region including the reactive site loop, inhibited the proteinases but the other one with the C-terminal sequence of the S-22 form lost the inhibitory activity. | |||||||
| 言語 | en | |||||||
| 出版者 | ||||||||
| 出版者 | The Kinki University Medical Association | |||||||
| 言語 | en | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | departmental bulletin paper | |||||||
| 出版タイプ | ||||||||
| 出版タイプ | AM | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||
| 収録物識別子 | ||||||||
| 収録物識別子タイプ | PISSN | |||||||
| 収録物識別子 | 03866092 | |||||||
| 開始ページ | ||||||||
| 開始ページ | 57 | |||||||
| 終了ページ | ||||||||
| 終了ページ | 65 | |||||||
| 書誌情報 |
en : ACTA MEDICA KINKI UNIVERSITY 巻 32, 号 2, p. 57-65, 発行日 2007-12 |
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