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分離ラット細胆管側肝細胞膜における抱合型ビリルビンの輸送機構について
https://kindai.repo.nii.ac.jp/records/2002630
https://kindai.repo.nii.ac.jp/records/20026307636fd83-05df-4d2c-a855-eb77df61f335
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AN00063584-19910625-0325.pdf (758.9 KB)
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| Item type | ☆紀要論文 / Departmental Bulletin Paper(1) | |||||||||
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| 公開日 | 2025-03-24 | |||||||||
| タイトル | ||||||||||
| タイトル | 分離ラット細胆管側肝細胞膜における抱合型ビリルビンの輸送機構について | |||||||||
| 言語 | ja | |||||||||
| タイトル | ||||||||||
| タイトル | Bilirubin diglucuronde transport by rat liver canalicular membrane vesicles | |||||||||
| 言語 | en | |||||||||
| 著者 |
小林, 宏明
× 小林, 宏明
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| 言語 | ||||||||||
| 言語 | jpn | |||||||||
| キーワード | ||||||||||
| 主題 | bilirubin diglucuronide, canalicular membrane vesicles, transport | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | departmental bulletin paper | |||||||||
| 出版タイプ | ||||||||||
| 出版タイプ | AM | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||
| 出版者 名前 | ||||||||||
| 出版者 | 近畿大学医学会 | |||||||||
| 言語 | ja | |||||||||
| bibliographic_information |
ja : 近畿大学医学雑誌 en : Medical Journal of Kinki University 巻 16, 号 2, p. 325-337, 発行日 1991-06-25 |
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| ISSN | ||||||||||
| 収録物識別子タイプ | PISSN | |||||||||
| 収録物識別子 | 03858367 | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | To elucidate the mechanism of bilirubin diglucuronide (BDG) excretion through the hepatocyte canalicular membrane, BDG transport was studied in isolated liver canalicular membrane vesicles by a rapid filtration method. BDG transport was not dependent on Na^+. BDG uptake was saturable (apparent Km for BDG=75μM and V_<MAX>=0.42n mol/mg protein×20s at 37℃) and was inhibited by BSP, but the inhibition by taurocholate was very small. By preincubation with unlabelled BDG, trans-stimulation of BDG transport could also be demonstrated. When the electrical potential difference across the membrane was altered by anion replacement, a more positive intravesicular potential stimulated, and a more negative potential inhibited BDG transport. Valinomycin-induced K^+ diffusion potential also enhanced BDG uptake. However, the use of inwardly directed inorganic ion gradients resulted in enhancement of [^3H]-BDG transport when Cl- was used. These findings provide direct evidence for the presence of a carrier mediated, sodium-independent and potential-sensitive BDG transport in rat liver canalicular membrane. In intact hepatocytes, the electrolical potential difference across the canalicylar membrane (about-30～-35mV) may provide the driving force BDG excretion. | |||||||||
| 言語 | en | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | 本文データはCiNiiから複製したものである。 | |||||||||
| 言語 | ja | |||||||||
