WEKO3
アイテム
マウス腹腔内マクロファージのザイモサン貪食について
https://kindai.repo.nii.ac.jp/records/2002612
https://kindai.repo.nii.ac.jp/records/2002612bba8f6e1-3abe-4a71-af6f-83f1655ae844
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AN00063584-19910325-0097.pdf (2.8 MB)
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| アイテムタイプ | ☆紀要論文 / Departmental Bulletin Paper(1) | |||||||||
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| 公開日 | 2025-03-19 | |||||||||
| タイトル | ||||||||||
| タイトル | マウス腹腔内マクロファージのザイモサン貪食について | |||||||||
| 言語 | ja | |||||||||
| タイトル | ||||||||||
| タイトル | Phagocytosis of zymosan by mouse peritoneal macrophages | |||||||||
| 言語 | en | |||||||||
| 著者 |
本田, 秀明
× 本田, 秀明
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| 言語 | ||||||||||
| 言語 | jpn | |||||||||
| キーワード | ||||||||||
| 主題 | macrophages, zymosan, phagocytosis, crater, cytoskeletons, β-glucan receptor | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | departmental bulletin paper | |||||||||
| 出版タイプ | ||||||||||
| 出版タイプ | AM | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||
| 出版者 名前 | ||||||||||
| 出版者 | 近畿大学医学会 | |||||||||
| 言語 | ja | |||||||||
| bibliographic_information |
ja : 近畿大学医学雑誌 en : Medical Journal of Kinki University 巻 16, 号 1, p. 97-115, 発行日 1991-03-25 |
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| ISSN | ||||||||||
| 収録物識別子タイプ | PISSN | |||||||||
| 収録物識別子 | 03858367 | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | The cell membrane and cytoskeletons of mouse peritoneal macrophages at various stages of phagocytosis of zymosan or latex particles were visualized by scanning electron microscopy (SEM). Zymosan particles were phagocytosed through the characteristic "craters" on the cell surface within 15 min of incubation. After further incubation for 15 min, the zymosan particles were engulfed into the cell and accumulated around the perinuclear region. On the other hand, latex particles were phagocytosed in the conventional style. This difference was confirmed by SEM of the cytoskeleton. At the early stage of phagocytosis, the craters appeared, and the cytoskeleton formed a basket-like shape around the zymosan particles from a distance. At the later stage of plagocytosis, zymosan particles were engulfed into the cell, and the cytoskeleton became attached to and redistributed radially around the zymosan particles at the perinuclear region. On the macrophages phagocytosing latex particles, the basket-like shape of cytoskeleton and the distance were not observed during the phagocytosis. To determine what kind of cytoskeleton distributes during the phagocytosis of zymosan, the cytoskeletons were double stained with rhodamine-phalloidin for F-actin and with indirect immunofluorescence for vimentin or actin binding proteins, α-actinin, calmodulin, clathrin, myosin or gelsolin, and observed with a phase contrast microscope equipped with epifluorescence optics. Within 15 min of incubation, F-actin accumulated and formed a ring around the zymosan particles at the peripheral region. Vimentin and actin binding proteins were also distributed in the same pattern as F-actin. After further incubation for 15 min, the ringed accumulation of cytoskeletons and actin binding proteins disappeared. The distribution of these cytoskeletons and actin binding proteins of macrophages phagocytosing latex particles was similar to that observed during zymosan phagocytosis. The effect of the major constituents of zymosan on zymosan binding to the cell membrane of macrophage at 4℃ was investigated. The yeast β-glucan and the algal β-glucan (laminarin) markedly indibited zymosan binding to the cell membrane and the inhibitory effect was does dependent, but mannan and N-acetyl-D-glucosamine did not affect it. These results suggest that, the receptor of macrophage for zymosan is the β-glucan receptor. | |||||||||
| 言語 | en | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | 本文データはCiNiiから複製したものである。 | |||||||||
| 言語 | ja | |||||||||
