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Endothelinの心筋肥大作用：ラット新生児培養心筋細胞を用いた研究
https://kindai.repo.nii.ac.jp/records/2002549
https://kindai.repo.nii.ac.jp/records/2002549f477a588-05cd-4e84-97f6-6b46563944dc
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AN00063584-19910925-0439.pdf (2.7 MB)
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| Item type | ☆紀要論文 / Departmental Bulletin Paper(1) | |||||||||
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| 公開日 | 2025-03-07 | |||||||||
| タイトル | ||||||||||
| タイトル | Endothelinの心筋肥大作用：ラット新生児培養心筋細胞を用いた研究 | |||||||||
| 言語 | ja | |||||||||
| タイトル | ||||||||||
| タイトル | Hypertrophic effect of endothelin : a study using rat neonatal cultured cardiac myocytes | |||||||||
| 言語 | en | |||||||||
| 著者 |
近藤, 宏和
× 近藤, 宏和
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| 言語 | ||||||||||
| 言語 | jpn | |||||||||
| キーワード | ||||||||||
| 主題 | cardiac hypertrophy, endothelin, cardiac myocytes, calcium influx, protein kinase C | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | departmental bulletin paper | |||||||||
| 出版タイプ | ||||||||||
| 出版タイプ | AM | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||
| 出版者 名前 | ||||||||||
| 出版者 | 近畿大学医学会 | |||||||||
| 言語 | ja | |||||||||
| bibliographic_information |
ja : 近畿大学医学雑誌 en : Medical Journal of Kinki University 巻 16, 号 3, p. 439-452, 発行日 1991-09-25 |
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| ISSN | ||||||||||
| 収録物識別子タイプ | PISSN | |||||||||
| 収録物識別子 | 03858367 | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | The induction of hypertrophy by endothelin, a newly discovered novel vasoconstrictor peptide, on cultured neonatal rat cardiac myocytes was investigated. Cultured cardiac myocytes were isolated by mechanical disaggregation and enzymatic digestion with trypsin from neonatal 1 to 3 days old Sprague-Dawley rat ventricles. These cells were cultured in a serum free condition. Endothelin-1, which is the most examined endothelin, was used in this study. Endothelin-1 enlarged the size of cardiac myocytes and induced well developed myofibrils as well as endoplasmic reticulum in the cytoplasm. Cell surface area, protein contents and [^<14>C] phenylalanine (Phe) uptake to the cells increased time-dependently 1, 2 and 3 days after the addition of endothelin-1. The [^<14>C] Phe uptake also increased dose-dependently by endothelin-1. The maximum response of endothelin-1 was obtained at around 10^<-8>M, and ED_<50> was about 2×10^<-10>M. These effects were attenuated significantly by nicardipine, a Ca^<2+> channel antagonist, and H-7, a specific protein kinase C inhibitor, but not by HA1004, a weak protein kinase C inhibitor. These results indicate that the mechanism by which endothelin induces hypertrophy of cultured cardiac myocytes is mediated by the increased extracellular Ca^<2+> influx and/or protein kinase C activation. | |||||||||
| 言語 | en | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | 本文データはCiNiiから複製したものである。 | |||||||||
| 言語 | ja | |||||||||
