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アイテム
抗DNA抗体遺伝子の突然変異，クラス転換と抗原親和性の変化
https://kindai.repo.nii.ac.jp/records/2002469
https://kindai.repo.nii.ac.jp/records/20024695669f2a8-4f57-440d-8569-50346ff6ac92
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AN00063584-19911225-0541.pdf (605.6 KB)
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| アイテムタイプ | ☆紀要論文 / Departmental Bulletin Paper(1) | |||||||||
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| 公開日 | 2025-02-26 | |||||||||
| タイトル | ||||||||||
| タイトル | 抗DNA抗体遺伝子の突然変異，クラス転換と抗原親和性の変化 | |||||||||
| 言語 | ja | |||||||||
| タイトル | ||||||||||
| タイトル | Isotype-switching and antigen-binding activity of anti-DNA antibodies in SLE model mice | |||||||||
| 言語 | en | |||||||||
| 著者 |
木下, 浩二
× 木下, 浩二
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| 言語 | ||||||||||
| 言語 | jpn | |||||||||
| キーワード | ||||||||||
| 主題 | systemic lupus erythematosus, NZB×NZWF1 mice, anti-DNA antiboby, immunoglobulin V_H gene, somatic mutation, affinity maturation | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | departmental bulletin paper | |||||||||
| 出版タイプ | ||||||||||
| 出版タイプ | AM | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||
| 出版者 名前 | ||||||||||
| 出版者 | 近畿大学医学会 | |||||||||
| 言語 | ja | |||||||||
| bibliographic_information |
ja : 近畿大学医学雑誌 en : Medical Journal of Kinki University 巻 16, 号 4, p. 541-552, 発行日 1991-12-25 |
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| ISSN | ||||||||||
| 収録物識別子タイプ | PISSN | |||||||||
| 収録物識別子 | 03858367 | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Autoimmune-prone NZB×NZWF1 (NZB/WF1) mice produce IgM anti-DNA antibodies in early life. However, at about 5-6 months of age IgG anti-DNA antibodies begin to develop and the titer exceeds that of IgM anti-DNA antibodies. To investigate the changes in V_H gene and affinity maturation associated with isotype-switching in such anti-DNA antibodies, we established several monoclonal anti-DNA antibodies of both IgM and IgG classes from one NZB/WF1 mouse and sequenced the V_H, D and J_H regions of the immunoglobulin heavy chain gene. Among them, one pair of IgM and IgG monoclonal antibodies was suggested to use the same ancestor V_H genes in the Q52 family. The V_H segment sequence of the IgM anti-DNA antibody clone was identical to that of a cloned NZW germline V_H gene except for the priming sites. Thus it was probably encoded by unmutated germline V_H genes. By contrast, the V_H segment of the IgG counterpart contained many somatically mutated sequences. The IgG anti-DNA antibody showed a higher DNA-binding activity than did the IgM antibody. This may be the first direct demonstration that the germline-encoded, low affinity IgM autoantibody could undergo somatic mutations associated with isotype-switching by an antigen-driven mechanism, which resulted in the acquisition of high affinity, as seen in normal immune responses. Such a mechanism may be responsible for the generation of pathogenic autoantibodies in autoimmune diseases. | |||||||||
| 言語 | en | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | 本文データはCiNiiから複製したものである。 | |||||||||
| 言語 | ja | |||||||||
