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ヒト甲状腺細胞のコラーゲン・ゲル内培養コロニーの形態と機能に関する研究
https://kindai.repo.nii.ac.jp/records/2002458
https://kindai.repo.nii.ac.jp/records/2002458cfd03de6-5096-4b8a-b44b-b1666b56ca84
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AN00063584-19920325-0095.pdf (2.9 MB)
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| Item type | ☆紀要論文 / Departmental Bulletin Paper(1) | |||||||||
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| 公開日 | 2025-02-19 | |||||||||
| タイトル | ||||||||||
| タイトル | ヒト甲状腺細胞のコラーゲン・ゲル内培養コロニーの形態と機能に関する研究 | |||||||||
| 言語 | ja | |||||||||
| タイトル | ||||||||||
| タイトル | Study of structural and functional on human thyroid cell colonies in collagen gel culture | |||||||||
| 言語 | en | |||||||||
| 著者 |
楠, 威志
× 楠, 威志
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| 言語 | ||||||||||
| 言語 | jpn | |||||||||
| キーワード | ||||||||||
| 主題 | collagen gel culture, thyroid tissue, morphological and functional examination | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | departmental bulletin paper | |||||||||
| 出版タイプ | ||||||||||
| 出版タイプ | AM | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||
| 出版者 名前 | ||||||||||
| 出版者 | 近畿大学医学会 | |||||||||
| 言語 | ja | |||||||||
| bibliographic_information |
ja : 近畿大学医学雑誌 en : Medical Journal of Kinki University 巻 17, 号 1, p. 95-111, 発行日 1992-03-25 |
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| ISSN | ||||||||||
| 収録物識別子タイプ | PISSN | |||||||||
| 収録物識別子 | 03858367 | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Thyroid cells obtained from 21 normal human thyroid tissues and 17 diseased thyroid tissues (1 Graves' disease, 4 follicular adenomas, 11 papillary carcinomas and 1 follicular carcinoma) were cultured in collagen gel, and then, morphological characteristics and thyroglobulin (Tg), triiodothyronine (T_3) and thyroxine (T_4) products were examined. In this study, the results were obtained as follows. Follicular adenoma cells and papillary carcinoma cells of the thyroid in collagen gel culture could survive for a longer term than in monolayer culture. In collagen gel culture, colonies revealed a three dimensional in vivo-like structure and demonstrated characteristic morphology depending on the orignal structure of the thyroid. In immunohistochemical analysis, Tg production was observed in colonies formed from 6 normal thyroid, 1 Graves' disease, 4 follicular adenomas, 8 papillary and 1 follicular carcinomas. T_3 production was observed in the colonies produced by 2 papillary and 1 follicular carcinomas. T_4 production was also observed in the colonies of 3 papillary and 1 follicular carcinomas. After addition of thyroid stimulating hormone (TSH) to medium in collagen gel culture, the colonies produced by normal thyroid gland cells showed differentiation, such as enlarged follicle and columnar epithelium. However there were no morphological changes in the colonies produced from diseased thyroid tissue. These findings suggested that collagen gel culture offered an in vivo-like environment to cultured cells and were a useful method for survival and differentiation of thyroid cells. | |||||||||
| 言語 | en | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | 本文データはCiNiiから複製したものである。 | |||||||||
| 言語 | ja | |||||||||
