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角膜上皮創傷治癒におけるフィブロネクチン受容体（インテグリン）の役割について
https://kindai.repo.nii.ac.jp/records/2002422
https://kindai.repo.nii.ac.jp/records/2002422e1f1debe-df70-4af8-96e7-5733fb4aeed4
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AN00063584-19920625-0223.pdf (1011.1 KB)
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| Item type | ☆紀要論文 / Departmental Bulletin Paper(1) | |||||||||
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| 公開日 | 2025-02-13 | |||||||||
| タイトル | ||||||||||
| タイトル | 角膜上皮創傷治癒におけるフィブロネクチン受容体（インテグリン）の役割について | |||||||||
| 言語 | ja | |||||||||
| タイトル | ||||||||||
| タイトル | Fibronectin receptors (integrin) in corneal epithelial wound healing | |||||||||
| 言語 | en | |||||||||
| 著者 |
村上, 純子
× 村上, 純子
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| 言語 | ||||||||||
| 言語 | jpn | |||||||||
| キーワード | ||||||||||
| 主題 | fibronectin receptor, fibronectin, wound healing, cornea, epithelium | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | departmental bulletin paper | |||||||||
| 出版タイプ | ||||||||||
| 出版タイプ | AM | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||
| 出版者 名前 | ||||||||||
| 出版者 | 近畿大学医学会 | |||||||||
| 言語 | ja | |||||||||
| bibliographic_information |
ja : 近畿大学医学雑誌 en : Medical Journal of Kinki University 巻 17, 号 2, p. 223-235, 発行日 1992-06-25 |
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| ISSN | ||||||||||
| 収録物識別子タイプ | PISSN | |||||||||
| 収録物識別子 | 03858367 | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Fibronectin has been reported to play an essential role in the attachment and migration of the corneal epithelial cells and has been successfully applied for the treatment of non-healing corneal epithelial disorders. Corneal epithelial cells have been suggested to possess fibronectin receptors on their cell surfaces. To investigate the role of fibronectin receptors during the wound healing of the cornea, chronological changes in the localization of fibronectin receptors were examined. The effects of the cell-adhesion-related peptide GRGDSP, an amino acid sequence of the cell binding domain of fibronectin, on the epithelial migration, and the effects of epidermal growth factor (EGF) on the regulation of fibronectin receptors activity were also studied. By immunofluorescence microscopy, the fibronectin receptors were observed predominantly in the basal cell layer and they were detected to a lesser degree in wing cell layer of the corneal epithelium. Shortly after an incision was made into the cornea, fibronectin receptors were observed in all the cell layers of the migrating epithelium apparently to cover the defects. When the cornea had healed, they were only observed in the basal layer strongly and in the wing cell layer weakly as in the normal cornea. The appearance and disappearance of fibronectin receptors in the corneal epithelial cells coordinated well with that of fibronectin which formed a temporary extracellular matrix. Thus fibronectin receptors and fibronectin seem to be very important for successful corneal epithelial wound healing. To further clarify the dynamic role of fibronectin receptors during epithelial wound healing, the effects of GRGDSP on the epithelial migration and attachment were studied. GRGDSP inhibited the epithelial migration on the organ-cultured cornea, fibronectin-stimulated epithelial migration, and epithelial cell attachment to the fibronectin matrix. These findings suggest a very important role of fibronectin receptors in the migration and attachment of corneal epithelial cells. EGF is known to accelerate corneal epithelial wound healing. EGF stimulated not only the epithelial migration on the organ-cultured cornea but also increased the number of cells attached to the fibronectin matrix. EGF-stimulated epithelial migration and cell attachment to the fibronectin matrix were both inhibited by GRGDSP. These findings strongly suggest that EGF stimulates the expression of fibronectin receptors in the corneal epithelial cells, and that a fibronectin-fibronectin receptor system plays a central role in the epithelial migration of the cornea, which leads to epithelial wound healing. | |||||||||
| 言語 | en | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | 本文データはCiNiiから複製したものである。 | |||||||||
| 言語 | ja | |||||||||
