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成人T細胞白血病由来因子の防御効果：腫瘍壊死因子の細胞障害への影響
https://kindai.repo.nii.ac.jp/records/2002297
https://kindai.repo.nii.ac.jp/records/2002297de28d88d-f829-48cc-b222-ed0b9f0bfa28
| 名前 / ファイル | ライセンス | アクション |
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AN00063584-19921225-0497.pdf (480.9 KB)
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| Item type | ☆紀要論文 / Departmental Bulletin Paper(1) | |||||||||
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| 公開日 | 2025-01-22 | |||||||||
| タイトル | ||||||||||
| タイトル | 成人T細胞白血病由来因子の防御効果：腫瘍壊死因子の細胞障害への影響 | |||||||||
| 言語 | ja | |||||||||
| タイトル | ||||||||||
| タイトル | Protective activity of adult T cell leukemia-derived factor (ADF) against tumor necrosis factor-dependent cytotoxicity on U937 cells | |||||||||
| 言語 | en | |||||||||
| 著者 |
松田, 光弘
× 松田, 光弘
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| 言語 | ||||||||||
| 言語 | jpn | |||||||||
| キーワード | ||||||||||
| 主題 | adult T cell leukemia-derived factor (ADF), thioredoxin (TRX), tumor necrosis factor (TNF), Fas antigen, radical scavenger | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | departmental bulletin paper | |||||||||
| 出版タイプ | ||||||||||
| 出版タイプ | AM | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||
| 出版者 名前 | ||||||||||
| 出版者 | 近畿大学医学会 | |||||||||
| 言語 | ja | |||||||||
| bibliographic_information |
ja : 近畿大学医学雑誌 en : Medical Journal of Kinki University 巻 17, 号 4, p. 497-505, 発行日 1992-12-25 |
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| ISSN | ||||||||||
| 収録物識別子タイプ | PISSN | |||||||||
| 収録物識別子 | 03858367 | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Adult T cell leukemia-derived factor (ADF) is a human homologue of thioredoxin (TRX) with many biological functions including interleukin-2 receptor (IL-2R) induction, growth promotion, thiol-dependent reducing activity, and radical scavenging activity. The regulatory effect of ADF on the cytotoxic activity of tumor necrosis factor (TNF) was examined by using a human histiocytic lymphoma cell line, U937. When U937 cells were preincubated with recombinant ADF (rADF) (0.1-100μg/ml) at 37℃ for 30 min, TNF-dependent cytotoxicity against U937 cells was markedly inhibited. This inhibitory effect was 95% in the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide assay (rADF 100 μg/ml) and 85% in the ^<51>Crreleasing assay (rADF 10μg/ml). After pretreatment of U937 cells with interferon-γ (IFN-γ) to augment the sensitivity to TNF, an inhibitory effect of rADF was also noted. U937 cells washed after preincubation with rADF also showed resistence to TNF-dependent cytotoxicity. This indicated that rADF inhibited the sensitivity of TNF receptors on U937 to TNF-dependent cytotoxicity rather than modifying TNF molecules. Scatchard analysis of TNF receptors on U937 cells using ^<125>I-TNF showed that rADF modulated neither the density of the receptors nor the affinity of the cell membrane significantly. rADF also reduced the cytotoxicity induced by anti-Fas IgM moAb which shows cytotoxicity quite similar to TNF. rADF (10μg/ml) reduced 90% of the cytotoxicity by anti-Fas IgM moAb, without a detectable change either in Fas Ag expression (MFI 58.1 vs 53.3) or in the degradation of anti-Fas IgM moAb as determined by flow cytometric analysis. These findings indicatd that the rADF-induced resistence to the cytotoxic effect of TNF and anti-Fas moAb was not related to the modulation of the TNF receptor or Fas Ag. | |||||||||
| 言語 | en | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | 本文データはCiNiiから複製したものである。 | |||||||||
| 言語 | ja | |||||||||
