{"created":"2025-01-17T01:50:38.993437+00:00","id":2002263,"links":{},"metadata":{"_buckets":{"deposit":"7426a56b-8e4f-4a28-9b63-d3ea0070f856"},"_deposit":{"created_by":42,"id":"2002263","owners":[42],"pid":{"revision_id":0,"type":"depid","value":"2002263"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:02002263","sets":["14:923:1477:1728433209792"]},"author_link":[],"item_2_biblio_info_21":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1993-03-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"82","bibliographicPageStart":"73","bibliographicVolumeNumber":"18","bibliographic_titles":[{"bibliographic_title":"近畿大学医学雑誌","bibliographic_titleLang":"ja"},{"bibliographic_title":"Medical Journal of Kinki University","bibliographic_titleLang":"en"}]}]},"item_2_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Delta bilirubin (Bδ) is a covalently albumin-bound bilirubin. A bilirubin binding amino acid residue of human albumin was identified using a large amount of Bδ fraction prepared from pooled icteric human serum. Bilirubin in pooled icteric human serum was first changed to an azoderivative by the addition of a diazo reagent to protect it from degradation. The azoderivative of Bδ (azoBδ) was prepared by affinity chromatography and high performance liquid chromatography (HPLC) using a preparative Micronex RP30 column. The BrCN-treated azo Bδ was digested with Achromobacter protease I and with Staphylococcal serine proteinase, sequentially. The fragmented peptides were fractionated by reversed phase HPLC using a μBondasphere column. A fraction which showed absorbance peaks both at 530 nm and at 215 nm was obtained and analyzed by a protein/peptide sequencer and an analyzer. The finally obtained peptide fraction was composed of amino acid residues 18 to 38 of human serum albumin. The detected molar amount of lysine 20 was about one third of that of the other amino acids which suggested the covalent bonding of bilirubin to lysine 20 of human serum albumin.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"本文データはCiNiiから複製したものである。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学医学会","subitem_publisher_language":"ja"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03858367","subitem_source_identifier_type":"PISSN"}]},"item_2_version_type_12":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kinki University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"村田, 尚美","creatorNameLang":"ja"},{"creatorName":"Murata, Naomi","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"AN00063584-19930325-0073.pdf","filesize":[{"value":"513.6 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://kindai.repo.nii.ac.jp/record/2002263/files/AN00063584-19930325-0073.pdf"},"version_id":"56e8b1e7-aeb8-48bb-8736-a3f010a3c917"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"delta bilirubin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"lysine","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"amino acid residue covalent bonding","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"デルタビリルビンにおけるビリルビン・アルブミン共有結合部位の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"デルタビリルビンにおけるビリルビン・アルブミン共有結合部位の検討","subitem_title_language":"ja"},{"subitem_title":"Covalent binding site of bilirubin to albumin molecule in serum delta bilirubin","subitem_title_language":"en"}]},"item_type_id":"2","owner":"42","path":["1728433209792"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2025-01-17"},"publish_date":"2025-01-17","publish_status":"0","recid":"2002263","relation_version_is_last":true,"title":["デルタビリルビンにおけるビリルビン・アルブミン共有結合部位の検討"],"weko_creator_id":"42","weko_shared_id":-1},"updated":"2025-02-06T03:15:34.728310+00:00"}