{"created":"2024-12-09T07:54:31.973470+00:00","id":2002116,"links":{},"metadata":{"_buckets":{"deposit":"41d18d5d-75a0-4ffa-8a16-9d4c83758b66"},"_deposit":{"created_by":41,"id":"2002116","owners":[41],"pid":{"revision_id":0,"type":"depid","value":"2002116"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:02002116","sets":["14:2667:1728522335637:1728528711661"]},"author_link":[],"control_number":"2002116","item_1693378644159":{"attribute_name":"研究分担者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"34419","affiliationNameIdentifierScheme":"kakenhi"}],"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kindai University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"安齋, 政幸","creatorNameLang":"ja"},{"creatorName":"Anzai, Masayuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"30454630","nameIdentifierScheme":"e-Rad"}]}]},"item_1693381679919":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"=資源タイプ識別子":"http://purl.org/coar/resource_type/c_93fc","resourcetype":"report","resourceuri":"http://purl.org/coar/resource_type/c_93fc"}]},"item_30001_bibliographic_information17":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicNumberOfPages":"5","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2023)","bibliographic_titleLang":"ja"}]}]},"item_30001_creator2":{"attribute_name":"研究代表者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"34419","affiliationNameIdentifierScheme":"kakenhi"}],"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kindai University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"黒坂, 哲","creatorNameLang":"ja"},{"creatorName":"Kurosaka, Satoshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"30625356","nameIdentifierScheme":"e-Rad_Researcher"}]}]},"item_30001_description8":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"マウス卵母細胞および初期胚においてアルギニル化されるタンパク質の検出に成功し、これらの中でN末端則経路を介して分解されることが予想できる11種類のタンパク質を発見することができた。しかしながら、研究期間内にこれらのタンパク質の動態を明らかにするには至らなかった。また、ATE1を欠失した卵母細胞を得ることができなかったため、アルギニル化が起こらない胚の解析を実施することができなかった。以上のように、本研究では当初の目的を達成することはできなかったが、ATE1が雌性配偶子形成に必須であることが示唆されたのは本研究の成果のひとつであり、今後の研究の発展が期待できるものであると考える。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"In this study, arginylated proteins were detected in mouse oocytes and preimplantation embryos by LC-MS/MS. Eleven of them were identified as the candidates of the targets of the proteolysis via N-end rule passwey. However, we were not able to do future analyses on the behavior or degradation of those proteins. We were not able to analyze the embryo derived from ATE1-depleted oocytes, because we failed to obtain ATE1-depleted oocytes from ATE1-CKO mice. The results obtained are not enough to achieve the purpose of this study. However, one of our results suggests that ATE1 is essential for female gametogenesis, providing interesting direction for the future study.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"研究分野：細胞生物学、発生生物学、生殖生物学","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_30001_file22":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-12-16"}],"filename":"21K05979seika.pdf","filesize":[{"value":"3.2 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://kindai.repo.nii.ac.jp/record/2002116/files/21K05979seika.pdf"},"version_id":"121925f1-b5a8-4d73-a7f4-757345b37ff7"}]},"item_30001_funding_reference15":{"attribute_name":"助成情報","attribute_value_mlt":[{"subitem_award_numbers":{"subitem_award_number":"21K05979","subitem_award_uri":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21K05979/"},"subitem_award_titles":[{"subitem_award_title":"初期胚のタンパク質分解におけるN末端則経路に関する研究","subitem_award_title_language":"ja"},{"subitem_award_title":"N-end rule pathway of protein degradation in early development.","subitem_award_title_language":"en"}],"subitem_funder_names":[{"subitem_funder_name":"独立行政法人日本学術振興会","subitem_funder_name_language":"ja"},{"subitem_funder_name":"Japan Society for the Promotion of Science","subitem_funder_name_language":"en"}]}]},"item_30001_language10":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_30001_subject7":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"N末端則経路","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"アルギニル化","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"初期胚","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_30001_title0":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"初期胚のタンパク質分解におけるN末端則経路に関する研究","subitem_title_language":"ja"},{"subitem_title":"N-end rule pathway of protein degradation in early development.","subitem_title_language":"en"}]},"item_30001_version_type12":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_title":"初期胚のタンパク質分解におけるN末端則経路に関する研究","item_type_id":"40001","owner":"41","path":["1728528711661"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-11-08"},"publish_date":"2024-11-08","publish_status":"0","recid":"2002116","relation_version_is_last":true,"title":["初期胚のタンパク質分解におけるN末端則経路に関する研究"],"weko_creator_id":"41","weko_shared_id":-1},"updated":"2024-12-16T08:48:21.763776+00:00"}