{"created":"2024-12-09T00:45:45.932046+00:00","id":2002092,"links":{},"metadata":{"_buckets":{"deposit":"218f1057-4e41-486b-b73e-4c8b0a07425c"},"_deposit":{"created_by":42,"id":"2002092","owners":[42],"pid":{"revision_id":0,"type":"depid","value":"2002092"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:02002092","sets":["14:2667:1728522335637:1728528848310"]},"author_link":[],"control_number":"2002092","item_1693381679919":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"=資源タイプ識別子":"http://purl.org/coar/resource_type/c_93fc","resourcetype":"report","resourceuri":"http://purl.org/coar/resource_type/c_93fc"}]},"item_30001_bibliographic_information17":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicNumberOfPages":"10","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2023)","bibliographic_titleLang":"ja"}]}]},"item_30001_creator2":{"attribute_name":"研究代表者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"34419","affiliationNameIdentifierScheme":"kakenhi"}],"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kindai University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"倉田, 淳志","creatorNameLang":"ja"},{"creatorName":"Kurata, Atsushi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"10416000","nameIdentifierScheme":"e-Rad_Researcher"}]}]},"item_30001_description8":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"腸内細菌は、細胞外膜小胞（MVs）と呼ばれる代謝物を介して、宿主の生体機能へ重大な影響を与える。本提案では、独自に発酵食品細菌や腸内細菌のMVsを対象に生理機能を解明して、新たな免疫賦活剤を開発する。研究の結果、乳酸菌やビフィズス菌のMVsについて物理化学的、生化学的、機能的な特性を解明できた。これらのMV画分から、免疫賦活作用を示すタンパク質を発見した。免疫細胞や腸管上皮細胞に対するこれらのMVsの作用機序を明らかにした。細胞外MVsを高生産する培地条件を見出した。ランダム変異導入により、細胞外MVsの高生産株を育種した。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"Membrane vesicles (MVs) are spherical bioactive nanoparticles that range in size between 50 and 100 nm and are naturally secreted by many bacteria. MVs released from gut bacteria are becoming increasingly recognized as having a significant impact on host biological functions. This proposal aims to elucidate the physiological functions of MVs derived from fermented-food bacteria and gut bacteria, and to develop immune adjuvants using these vesicles. We were able to elucidate the physicochemical, biochemical, and functional properties of MVs from L. plantarum and B. infantis. We were able to elucidate the mechanisms by which these MVs act on immune cells and intestinal epithelial cells. We identified proteins with an immune-stimulating activity from these MV fractions. We developed a culture medium that allows for the high-yield production of MVs. We developed high-producing strains of extracellular MVs through random mutagenesis.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"研究分野：応用微生物学","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_30001_file22":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"21K05352seika.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://kindai.repo.nii.ac.jp/record/2002092/files/21K05352seika.pdf"},"version_id":"23995b71-bb90-4bca-a8f7-da6fffd8da45"}]},"item_30001_funding_reference15":{"attribute_name":"助成情報","attribute_value_mlt":[{"subitem_award_numbers":{"subitem_award_number":"21K05352","subitem_award_uri":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21K05352/"},"subitem_award_titles":[{"subitem_award_title":"発酵食品細菌や腸内細菌によって生産される膜小胞の生理機能の解明と応用","subitem_award_title_language":"ja"},{"subitem_award_title":"Elucidation o the physiological functions of membrane vesicles produced by fermented food-derived bacteria and their application","subitem_award_title_language":"en"}],"subitem_funder_names":[{"subitem_funder_name":"独立行政法人日本学術振興会","subitem_funder_name_language":"ja"},{"subitem_funder_name":"Japan Society for the Promotion of Science","subitem_funder_name_language":"en"}]}]},"item_30001_language10":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_30001_subject7":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"細胞外膜小胞","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"腸内細菌","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"抗体","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"蛍光顕微鏡観察","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"免疫賦活作用","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"タンパク質","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"サイトカイン","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"Toll様受容体","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_30001_title0":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"発酵食品細菌や腸内細菌によって生産される膜小胞の生理機能の解明と応用","subitem_title_language":"ja"},{"subitem_title":"Elucidation o the physiological functions of membrane vesicles produced by fermented food-derived bacteria and their application","subitem_title_language":"en"}]},"item_30001_version_type12":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_title":"発酵食品細菌や腸内細菌によって生産される膜小胞の生理機能の解明と応用","item_type_id":"40001","owner":"42","path":["1728528848310"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-11-08"},"publish_date":"2024-11-08","publish_status":"0","recid":"2002092","relation_version_is_last":true,"title":["発酵食品細菌や腸内細菌によって生産される膜小胞の生理機能の解明と応用"],"weko_creator_id":"42","weko_shared_id":-1},"updated":"2025-01-07T01:16:02.456593+00:00"}