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骨肉腫株化細胞(NY)の線溶系因子に及ぼす骨吸収因子の影響
https://kindai.repo.nii.ac.jp/records/2002046
https://kindai.repo.nii.ac.jp/records/20020465c1628d5-3c97-44e8-95a7-41e1524149cd
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AN00063584-19931225-0547.pdf (786.2 KB)
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| Item type | ☆紀要論文 / Departmental Bulletin Paper(1) | |||||||||
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| 公開日 | 2024-11-22 | |||||||||
| タイトル | ||||||||||
| タイトル | 骨肉腫株化細胞(NY)の線溶系因子に及ぼす骨吸収因子の影響 | |||||||||
| 言語 | ja | |||||||||
| タイトル | ||||||||||
| タイトル | Effect of bone resorbing factors on fibrinolytic factors in osteosarcoma cell line (NY) | |||||||||
| 言語 | en | |||||||||
| 著者 |
野中, 藤吾
× 野中, 藤吾
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| 言語 | ||||||||||
| 言語 | jpn | |||||||||
| キーワード | ||||||||||
| 主題 | bone resorption, pericellular proteolysis, urokinase-type plasminogen activator (u-PA), u-PA receptor (u-PAR), plasminogen activator inhibitor-1 (PAI-1), osteoblast-like cell | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | departmental bulletin paper | |||||||||
| 出版タイプ | ||||||||||
| 出版タイプ | AM | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||
| 出版者 名前 | ||||||||||
| 出版者 | 近畿大学医学会 | |||||||||
| 言語 | ja | |||||||||
| bibliographic_information |
ja : 近畿大学医学雑誌 en : Medical Journal of Kinki University 巻 18, 号 4, p. 547-561, 発行日 1993-12-25 |
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| ISSN | ||||||||||
| 収録物識別子タイプ | PISSN | |||||||||
| 収録物識別子 | 03858367 | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Recently, the specific receptor for the urokinase-type plasminogen activator (u-PA) receptor, u-PAR was demonstrated in several cell types. u-PA bound to u-PAR is closely associated with pericellular proteolysis. In the present study, the effect of bone resorbing factors on the pericellular fibrinolytic system of osteosarcoma NY cells was investigated. NY cells secrete single-chain u-PA (scu-PA) which is converted to two-chain u-PA by several proteases such as plasmin Parathyroid hormone (PTH), prostaglandine E_2 (PGE_2) or tumor necrosis factor α (TNF-α) enhanced the secretion of u-PA antigen and u-PA activity, and suppressed the secretion of plasminogen activator inhibitor-1 (PAI-1) antigen to the conditioned medium. The former two factors also increased u-PA antigen and u-PA activity on the cell surface. Transforming growth factor β (TGF-β) enhanced the u-PA antigen in the conditioned medium, but its activity was suppressed due to the increased secretion of PAI-1. ^<125>I-DFP-u-PA bound specifically to NY cells and the binding analysis revealed the presence of a single class of binding sites for u-PA. PTH and PGE_2 increased Bmax by 1.4-fold and 1.7-fold, respectively, and enhanced the u-PAR mRNA levels by 1.4-fold and 2.4-fold, respectively. However, TGF-β did not alter either the Kd or u-PAR mRNA level. Thus, pericellular fibrinolytic activity by u-PA/u-PAR and PAI-1 is modulated by bone resorbing factors. | |||||||||
| 言語 | en | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | 本文データはCiNiiから複製したものである。 | |||||||||
| 言語 | ja | |||||||||
