{"created":"2024-11-15T00:50:08.076269+00:00","id":2002001,"links":{},"metadata":{"_buckets":{"deposit":"4afb343d-df44-4051-8326-1b367d6bd9b6"},"_deposit":{"created_by":42,"id":"2002001","owners":[42],"pid":{"revision_id":0,"type":"depid","value":"2002001"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:02002001","sets":["14:923:1477:1728432905107"]},"author_link":[],"item_2_biblio_info_21":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1994-03-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"45","bibliographicPageStart":"33","bibliographicVolumeNumber":"19","bibliographic_titles":[{"bibliographic_title":"近畿大学医学雑誌","bibliographic_titleLang":"ja"},{"bibliographic_title":"Medical Journal of Kinki University","bibliographic_titleLang":"en"}]}]},"item_2_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"The target antigen, 54 kD glycoprotein (gp54), involved in the anti-tubular basement membrane (anti-TBM) antibody-mediated tubulo-interstitial nephritis, and localized dominantly to the basement membrane of proximal tubules was characterized. gp54 was isolated from collagenase-digested (CD) bovine TBM by column chromatagraphy and further elution from blotted membranes. Amino acid analysis showed that the gp54 was non-collagenous in nature. The NH_2-terminal amino acid sequencing of gp54 revealed that the gp54 was a unique basement membrane component. The nephritogenicity of gp54 was demonstrated by immunizing guinea pigs (strain XIII) with purified gp54, and producing anti-gp54 antibody and tubulo-interstitial nephritis. Anti-gp54 antibody, affinity-purified from sera of patients with anti-TBM nephritis, anti-gp54 peptide antibody, and antibody eluted from guinea pigs immunized with gp54 bound to 54 kD and 48 kD components of human CD-TBM. mRNA was prepared from rabbit kidneys and concentrated by the sucrose density gradient centrifugation method. ^<35>S-Me-thionine-labeled proteins translated from the rabbit renal mRNA were incubated and immunoprecipitated with anti-gp54 antibody, revealing mainly 54 kD and 48 kD components. A competition test using purified gp54 showed that anti-gp54 antibody was cross-reactive with 48kD component. Dot blot hybridization with an oligonucleotide probe for gp54 revealed a positive signal in cDNA libraries mada from adult and fetal kidneys. These findings indicate that 54kD and 48kD have similar antigenecity, but are encoded by different mRNA.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"本文データはCiNiiから複製したものである。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学医学会","subitem_publisher_language":"ja"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03858367","subitem_source_identifier_type":"PISSN"}]},"item_2_version_type_12":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kinki University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"宮里, 裕典","creatorNameLang":"ja"},{"creatorName":"Miyazato, Hirofumi","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"AN00063584-19940325-0033.pdf","filesize":[{"value":"944.3 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://kindai.repo.nii.ac.jp/record/2002001/files/AN00063584-19940325-0033.pdf"},"version_id":"b8923836-201f-46b0-9717-247e68b9f9af"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"tubulo-interstitial nephritis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"anti-tubular basement membrane antibody","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"mRNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"間質性腎炎惹起性抗原の分離・精製およびmRNA","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"間質性腎炎惹起性抗原の分離・精製およびmRNA","subitem_title_language":"ja"},{"subitem_title":"Isolation and molecular characterization of the tubular basement membrane antigen associated with tubulo-interstitial nephritis","subitem_title_language":"en"}]},"item_type_id":"2","owner":"42","path":["1728432905107"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-11-15"},"publish_date":"2024-11-15","publish_status":"0","recid":"2002001","relation_version_is_last":true,"title":["間質性腎炎惹起性抗原の分離・精製およびmRNA"],"weko_creator_id":"42","weko_shared_id":-1},"updated":"2024-12-09T05:29:09.853364+00:00"}