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間質性腎炎惹起性抗原の分離・精製およびmRNA
https://kindai.repo.nii.ac.jp/records/2002001
https://kindai.repo.nii.ac.jp/records/2002001131cedd2-038e-4e82-bbb0-3ef63682c46e
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AN00063584-19940325-0033.pdf (944.3 KB)
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| Item type | ☆紀要論文 / Departmental Bulletin Paper(1) | |||||||||
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| 公開日 | 2024-11-15 | |||||||||
| タイトル | ||||||||||
| タイトル | 間質性腎炎惹起性抗原の分離・精製およびmRNA | |||||||||
| 言語 | ja | |||||||||
| タイトル | ||||||||||
| タイトル | Isolation and molecular characterization of the tubular basement membrane antigen associated with tubulo-interstitial nephritis | |||||||||
| 言語 | en | |||||||||
| 著者 |
宮里, 裕典
× 宮里, 裕典
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| 言語 | ||||||||||
| 言語 | jpn | |||||||||
| キーワード | ||||||||||
| 主題 | tubulo-interstitial nephritis, anti-tubular basement membrane antibody, mRNA | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | departmental bulletin paper | |||||||||
| 出版タイプ | ||||||||||
| 出版タイプ | AM | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||
| 出版者 名前 | ||||||||||
| 出版者 | 近畿大学医学会 | |||||||||
| 言語 | ja | |||||||||
| bibliographic_information |
ja : 近畿大学医学雑誌 en : Medical Journal of Kinki University 巻 19, 号 1, p. 33-45, 発行日 1994-03-25 |
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| ISSN | ||||||||||
| 収録物識別子タイプ | PISSN | |||||||||
| 収録物識別子 | 03858367 | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | The target antigen, 54 kD glycoprotein (gp54), involved in the anti-tubular basement membrane (anti-TBM) antibody-mediated tubulo-interstitial nephritis, and localized dominantly to the basement membrane of proximal tubules was characterized. gp54 was isolated from collagenase-digested (CD) bovine TBM by column chromatagraphy and further elution from blotted membranes. Amino acid analysis showed that the gp54 was non-collagenous in nature. The NH_2-terminal amino acid sequencing of gp54 revealed that the gp54 was a unique basement membrane component. The nephritogenicity of gp54 was demonstrated by immunizing guinea pigs (strain XIII) with purified gp54, and producing anti-gp54 antibody and tubulo-interstitial nephritis. Anti-gp54 antibody, affinity-purified from sera of patients with anti-TBM nephritis, anti-gp54 peptide antibody, and antibody eluted from guinea pigs immunized with gp54 bound to 54 kD and 48 kD components of human CD-TBM. mRNA was prepared from rabbit kidneys and concentrated by the sucrose density gradient centrifugation method. ^<35>S-Me-thionine-labeled proteins translated from the rabbit renal mRNA were incubated and immunoprecipitated with anti-gp54 antibody, revealing mainly 54 kD and 48 kD components. A competition test using purified gp54 showed that anti-gp54 antibody was cross-reactive with 48kD component. Dot blot hybridization with an oligonucleotide probe for gp54 revealed a positive signal in cDNA libraries mada from adult and fetal kidneys. These findings indicate that 54kD and 48kD have similar antigenecity, but are encoded by different mRNA. | |||||||||
| 言語 | en | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | 本文データはCiNiiから複製したものである。 | |||||||||
| 言語 | ja | |||||||||
