{"created":"2024-11-08T07:04:20.632543+00:00","id":2001973,"links":{},"metadata":{"_buckets":{"deposit":"e92cd688-779b-4c44-aea5-9bd056013107"},"_deposit":{"created_by":42,"id":"2001973","owners":[42],"pid":{"revision_id":0,"type":"depid","value":"2001973"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:02001973","sets":["14:2667:1728522335637:1728528474285"]},"author_link":[],"item_1693378644159":{"attribute_name":"研究分担者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"34419","affiliationNameIdentifierScheme":"kakenhi"}],"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kindai University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"小澤, 大作","creatorNameLang":"ja"},{"creatorName":"Ozawa, Daisaku","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"60554524","nameIdentifierScheme":"e-Rad"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"34419","affiliationNameIdentifierScheme":"kakenhi"}],"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kindai University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"上山, 盛夫","creatorNameLang":"ja"},{"creatorName":"Ueyama Morio","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"20386593","nameIdentifierScheme":"e-Rad"}]}]},"item_1693381679919":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"=資源タイプ識別子":"http://purl.org/coar/resource_type/c_93fc","resourcetype":"report","resourceuri":"http://purl.org/coar/resource_type/c_93fc"}]},"item_30001_bibliographic_information17":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicNumberOfPages":"20","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2023)","bibliographic_titleLang":"ja"}]}]},"item_30001_creator2":{"attribute_name":"研究代表者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"34419","affiliationNameIdentifierScheme":"kakenhi"}],"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kindai University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"永井, 義隆","creatorNameLang":"ja"},{"creatorName":"Nagai, Yoshitaka","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"60335354","nameIdentifierScheme":"e-Rad_Researcher"}]}]},"item_30001_description8":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"本研究では、ポリグルタミン（PolyQ）病、筋萎縮性側索硬化症（ALS）などの神経変性疾患における原因蛋白質の液-液相分離（LLPS）の制御破綻による凝集メカニズムの解明を目的として、１）Thio-PolyQとTDP-43-MBPの試験管内実験系を樹立し、両蛋白質のLLPS・凝集を阻害する化合物Xを同定した。２）培養細胞において、DCTN1ノックダウンなどの微小管輸送障害によりTDP-43のストレス顆粒からの解離が遅延し、さらにALSモデルショウジョウバエにおいて、DCTN1など関連分子のノックダウンによりTDP-43凝集体および神経変性が増悪することを明らかにした。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"In this study, we explored the regulatory mechanisms of LLPS leading to pathological protein aggregation in neurodegenerative diseases. We identified compound X that can inhibit LLPS and aggregation of both Thio-PolyQ and TDP-43-MBP. We also found that disruption of microtubule-dependent transport by DCTN1 deficiency delays the disassembly of TDP-43 stress granules in cells, and accelerates TDP-43 aggregation and neurodegeneration in ALS Drosophila models.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"研究分野：神経内科学","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_30001_file22":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"21H02840seika.pdf","filesize":[{"value":"137.9 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://kindai.repo.nii.ac.jp/record/2001973/files/21H02840seika.pdf"},"version_id":"4b766c0c-7d9c-4f72-9cfe-15be6cacbbf0"}]},"item_30001_funding_reference15":{"attribute_name":"助成情報","attribute_value_mlt":[{"subitem_award_numbers":{"subitem_award_number":"21H02840","subitem_award_uri":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21H02840/"},"subitem_award_titles":[{"subitem_award_title":"神経変性疾患における液-液相分離の制御破綻による蛋白質凝集メカニズムの解明","subitem_award_title_language":"ja"},{"subitem_award_title":"Elucidation of regulation mechanisms of LLPS leading to pathological protein aggregation in neurodegenerative diseases","subitem_award_title_language":"en"}],"subitem_funder_names":[{"subitem_funder_name":"独立行政法人日本学術振興会","subitem_funder_name_language":"ja"},{"subitem_funder_name":"Japan Society for the Promotion of Science","subitem_funder_name_language":"en"}]}]},"item_30001_language10":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_30001_subject7":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"脳神経疾患","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"蛋白質","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"液－液相分離","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"非膜性オルガネラ","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"アミロイド","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"凝集体","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"微小管輸送","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"ショウジョウバエ","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_30001_title0":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"神経変性疾患における液-液相分離の制御破綻による蛋白質凝集メカニズムの解明","subitem_title_language":"ja"},{"subitem_title":"Elucidation of regulation mechanisms of LLPS leading to pathological protein aggregation in neurodegenerative diseases","subitem_title_language":"en"}]},"item_30001_version_type12":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_title":"神経変性疾患における液-液相分離の制御破綻による蛋白質凝集メカニズムの解明","item_type_id":"40001","owner":"42","path":["1728528474285"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-11-08"},"publish_date":"2024-11-08","publish_status":"0","recid":"2001973","relation_version_is_last":true,"title":["神経変性疾患における液-液相分離の制御破綻による蛋白質凝集メカニズムの解明"],"weko_creator_id":"42","weko_shared_id":-1},"updated":"2024-12-09T05:52:19.527914+00:00"}