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アンジオテンシンIIのラット血管平滑筋細胞肥大作用：c-mycアンチセンス オリゴデオキシヌクレオチドを用いた実験的研究
https://kindai.repo.nii.ac.jp/records/2001838
https://kindai.repo.nii.ac.jp/records/20018382a837b23-35ee-4a10-b36c-bc3ba3e22466
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AN00063584-19950625-0025.pdf (1.0 MB)
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| Item type | ☆紀要論文 / Departmental Bulletin Paper(1) | |||||||||
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| 公開日 | 2024-10-09 | |||||||||
| タイトル | ||||||||||
| タイトル | アンジオテンシンIIのラット血管平滑筋細胞肥大作用：c-mycアンチセンス オリゴデオキシヌクレオチドを用いた実験的研究 | |||||||||
| 言語 | ja | |||||||||
| タイトル | ||||||||||
| タイトル | Hypertrophic effect of angiotensin II on vascular smooth muscle cells : An experimental study using antisense oligodeoxynucleotides for c-myc mRNA | |||||||||
| 言語 | en | |||||||||
| 著者 |
鴨井, 公司
× 鴨井, 公司
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| 言語 | ||||||||||
| 言語 | jpn | |||||||||
| キーワード | ||||||||||
| 主題 | angiotensin II, vascular smooth muscle cell, hypertrophy, c-myc, antisense oligodeoxynucleotides | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | departmental bulletin paper | |||||||||
| 出版タイプ | ||||||||||
| 出版タイプ | AM | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||
| 出版者 名前 | ||||||||||
| 出版者 | 近畿大学医学会 | |||||||||
| 言語 | ja | |||||||||
| bibliographic_information |
ja : 近畿大学医学雑誌 en : Medical Journal of Kinki University 巻 20, 号 1, p. 25-35, 発行日 1995-06-25 |
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| ISSN | ||||||||||
| 収録物識別子タイプ | PISSN | |||||||||
| 収録物識別子 | 03858367 | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | The study investigated whether the expression of mRNA for c-myc is essential in VSMC hypertrophy induced by Angiotensin II (Ang. II) and explored the possibilty of inhibiting their growth using antisense oligodeoxynucleotides (ODN) for c-myc mRNA. We used antisense ODN specific for c-myc mRNA from exon 2 (1015-1029). Cultured vascular smooth muscle cells (VSMC) were isolated from the thoracic aorta of Sprague-Dawley rats (male, 7-8 weeks) by the explant method. These cells (passage 4-7) were brought to the subconfluent state, and then the medium was changed to a low serum concentration. After 48 hours, the VSMC were stimulated by 10^<-5> M Ang. II with or without antisense ODN. Ang. II increased the uptake of [^3H]thymidine and [^3H]leucine by the VSMC. After 5 days, the cell number was constant. Ang. II increased [^3H]thymidine uptake to VSMC, which was specifically inhibited by antisense ODN for c-myc mRNA in a dose-dependent manner. More than 5 pM of antisense ODN produced significant inhibition, and a maximal inhibitory effect of 75% at a concentration of 500 pM. However, sense ODN did not have any effect. The addition of c-myc antisense ODN 6 hours after Ang. II stimulation did not decrease [^3H]thymidine uptake into VSMC, because c-myc was an immediate early response gene induced by Ang. II stimulation. Similarly, c-myc antisense ODN decreased the [^3H]leucine uptake into VSMC induced by Ang. II. These results indicate that c-myc is necessary for VSMC hypertrophy induced by Ang. II, using antisense ODN to inhibit specific gene expression. | |||||||||
| 言語 | en | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | 本文データはCiNiiから複製したものである。 | |||||||||
| 言語 | ja | |||||||||
