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角膜実質細胞によるコラーゲン分解の調節機序について
https://kindai.repo.nii.ac.jp/records/2001782
https://kindai.repo.nii.ac.jp/records/2001782aa0d6a27-cfdd-4020-bd8e-f2d25da9e770
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AN00063584-19940625-0243.pdf
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| Item type | ☆紀要論文 / Departmental Bulletin Paper(1) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2024-09-04 | |||||||||
| タイトル | ||||||||||
| タイトル | 角膜実質細胞によるコラーゲン分解の調節機序について | |||||||||
| 言語 | ja | |||||||||
| タイトル | ||||||||||
| タイトル | Regulatory mechanism of collagen degradation by keratocytes | |||||||||
| 言語 | en | |||||||||
| 著者 |
岡本, 純之助
× 岡本, 純之助
|
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| 言語 | ||||||||||
| 言語 | jpn | |||||||||
| キーワード | ||||||||||
| 主題 | collagen degradation, keratocytes, collagenase, interleukin 1 (IL-1), interleukin 1 receptor antagonist (IL-1ra), cytochalasin B, chloroquine | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | departmental bulletin paper | |||||||||
| 版 | ||||||||||
| 出版タイプ | AM | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||
| 出版者 名前 | ||||||||||
| 出版者 | 近畿大学医学会 | |||||||||
| 言語 | ja | |||||||||
| 書誌情報 |
ja : 近畿大学医学雑誌 en : Medical Journal of Kinki University 巻 19, 号 2, p. 243-256, 発行日 1994-06-25 |
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| ISSN | ||||||||||
| 収録物識別子タイプ | PISSN | |||||||||
| 収録物識別子 | 03858367 | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Excessive degradation of stromal collagen fibrils occurs in corneal ulceration. Keratocytes (corneal fibroblasts) seem to play a primary role in collagen degradation in corneal ulceration. However, the mechanisms of collagen degradation in corneal ulceration have not been fully understood. To understand the pathobiology of corneal ulceration, I developed a new in-vitro model in which keratocytes were cultured in a collagen matrix three dimensionally. Using this model, I searched for any factors which might regulate collagen degradation by keratocytes. Collagen degradation was measured by the amount of hydroxyproline in the hydrolysate. Collagen degradation by keratocytes depended on the number of cells, the concentration of collagen and the period of incubation. Among various cytokines and growth factors such as epidermal growth factor (EGF), fibroblast growth factor (FGF), insulin-like growth factor 1 (IGF-1), interleukin 1 (IL-1) and interleukin 6 (IL-6) only IL-1 was found to enhance collagen degradation by keratocytes in a dose dependent manner. Interleukin 1 receptor antagonist (IL-1ra) antagonized the stimulatory effect of IL-1 on collagen degradation by keratocytes. IL-1 also stimulated collagenase synthesis by keratocytes. When phagocytic activity or intracellular lysosomal enzyme activities were inhibited by the addition of cytochalasin B or chloroquine, collagen degradation showed a decrease in the presence of IL-1. These findings suggested that both extracellular and intracellular collagen degradation played important roles in the stimulatory effect of IL-1 on collagen degradation by keratocytes. | |||||||||
| 言語 | en | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | 本文データはCiNiiから複製したものである。 | |||||||||
| 言語 | ja | |||||||||
