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Flow cytometryを用いた細胞周期解析におけるbackground補正によるS phase fractionの計測：大腸癌の悪性度に関する検討
https://kindai.repo.nii.ac.jp/records/2001569
https://kindai.repo.nii.ac.jp/records/2001569f87f36e7-72f4-4c9a-aa59-f0eba769651a
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AN00063584-19951225-0243.pdf
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| Item type | ☆紀要論文 / Departmental Bulletin Paper(1) | |||||||||||||||||
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| 公開日 | 2024-07-12 | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | Flow cytometryを用いた細胞周期解析におけるbackground補正によるS phase fractionの計測：大腸癌の悪性度に関する検討 | |||||||||||||||||
| 言語 | ja | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | The background compensated S phase fraction measurement for cell cycle analysis using flow cytometry : A study on malignant potential in colorectal carcinoma | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 著者 |
船井, 貞往
× 船井, 貞往
× 黒岡, 一仁
× 進藤, 勝久
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| 言語 | ||||||||||||||||||
| 言語 | jpn | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 主題 | flow cytometry, S phase fraction, background compensation, malignant potential, colorectal carcinoma | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||||||||
| 版 | ||||||||||||||||||
| 出版タイプ | AM | |||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||||||
| 出版者 名前 | ||||||||||||||||||
| 出版者 | 近畿大学医学会 | |||||||||||||||||
| 言語 | ja | |||||||||||||||||
| 書誌情報 |
ja : 近畿大学医学雑誌 en : Medical Journal of Kinki University 巻 20, 号 2, p. 243-256, 発行日 1995-12-25 |
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| ISSN | ||||||||||||||||||
| 収録物識別子タイプ | PISSN | |||||||||||||||||
| 収録物識別子 | 03858367 | |||||||||||||||||
| 内容記述 | ||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||
| 内容記述 | MultiCycle™ software (Multi-Cycle) is a computer program used to analyze cellular DNA content. This program is useful in mathematically compensating background such as debris, aggregation and sliced nuclei that exist in DNA histograms. Multi-Cycle was used to evaluate S phase fraction (SPF) in colorectal carcinoma cell lines after bromodeoxyuridine labeling. The bromodeoxyuridine labeling index and the SPF were approximate. Also when comparing the same colorectal carcinoma cell lines using Multi-Cycle and a general software, Sum of broadened rectangles model (SOBR), the SPF calculation using Multi-Cycle was significantly lower. To evaluate patient cancers, the SPF was determined using paraffin-embedded materials of colorectal carcinoma. Multi-Cycle showed a low coefficient of variation and SPF. The SOBR was not able to analyze the DNA histogram because of the background. Consequently, Multi-Cycle is indicated as a powerful tool for DNA and cell cycle analysis. When observing colorectal carcinoma from clinicopathological factors, there were no correlations between histological differentiation and DNA ploidy patterns. However, for the clinicopathological factors of invasion beyond the proper muscle, vessel permeation and positive lymph node and liver metastasis, a high DNA aneuploidy was found. Also when comparing the SPF in DNA diploidy and DNA aneuploidy, the proliferative activity of DNA aneuploidy was significantly higher. A high SPF rate was found in DNA aneuploid cancers less than 5.5 cm in size, an invasion beyond the proper muscle and showing positive lymph node metastasis. Furthermore, as the mutual relations between DNA index and SPF have been recognized, DNA aneuploidy can be said to have high malignant potential in the assessment of proliferative activity. Therefore, SPF calculation using Multi-Cycle is recognized as an important indicator in elucidating the malignant potential of colorectal cancer. | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 内容記述 | ||||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||||
| 内容記述 | 本文データはCiNiiから複製したものである。 | |||||||||||||||||
| 言語 | ja | |||||||||||||||||
