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Arduino UNOと電気リレーを使用した細胞電気穿孔システム
https://kindai.repo.nii.ac.jp/records/2001324
https://kindai.repo.nii.ac.jp/records/200132441b33f15-841e-4d76-9678-e745d768fd48
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AA12315376-20240315-0051.pdf
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| Item type | 紀要論文 / departmental bulletin paper(1) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2024-05-31 | |||||||||
| タイトル | ||||||||||
| タイトル | Arduino UNOと電気リレーを使用した細胞電気穿孔システム | |||||||||
| 言語 | ja | |||||||||
| タイトル | ||||||||||
| タイトル | Testing the application of the Arduino UNO and an electric relay for cellular electroporation | |||||||||
| 言語 | en | |||||||||
| 作成者 |
黄, 文敬
× 黄, 文敬
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| 言語 | ||||||||||
| 言語 | eng | |||||||||
| キーワード | ||||||||||
| 主題 | Cellular electroporation system, high-voltage waveform, electric field | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Electroporation is an effective method for inducing poration in the cell membrane and delivering macromolecules into cells in the fields of bioengineering and biomedicine. However, since the internal structures of commercially available power supply devices are not disclosed to the public and exhibit low operability, this study aims to develop an electroporation system utilizing Arduino UNO and an electric relay. This system facilitates the easy generation of waveforms by toggling a pin on and off for specified durations (Arduino programming). In this study, a DC-DC converter was employed as the power supply (DC 45-390 V). The ON/OFF time of the electrical relay was controlled by Arduino UNO for waveform generation. For instance, a waveform with an ON/OFF time of 10/500 ms can be generated. When this waveform was applied to a 3-mm gap electroporation cuvette, an electric field with a field strength of 200 V/cm was produced. Using this setup, 40-kDa fluorescein isothiocyanate (FITC)-dextran was successfully delivered into NIH/3T3 cells, indicating poration in the cell membrane. Thus, this study has established a cell electroporation system with high operability. | |||||||||
| 言語 | en | |||||||||
| 出版者 | ||||||||||
| 出版者 | 近畿大学工業高等専門学校 | |||||||||
| 言語 | ja | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | departmental bulletin paper | |||||||||
| 出版タイプ | ||||||||||
| 出版タイプ | AM | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||
| 収録物識別子 | ||||||||||
| 収録物識別子タイプ | PISSN | |||||||||
| 収録物識別子 | 18824374 | |||||||||
| 書誌情報 |
ja : 近畿大学工業高等専門学校研究紀要 en : Research reports Kindai University Technical College 号 17, p. 51-54, 発行日 2024-03-15 |
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