{"created":"2024-05-08T07:10:08.535600+00:00","id":2001217,"links":{},"metadata":{"_buckets":{"deposit":"195d7c91-a06a-4b96-a079-42f08e54024b"},"_deposit":{"created_by":42,"id":"2001217","owners":[42],"pid":{"revision_id":0,"type":"depid","value":"2001217"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:02001217","sets":["14:923:1477:1714622708958"]},"author_link":[],"control_number":"2001217","item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1997-06-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"18","bibliographicPageStart":"7","bibliographicVolumeNumber":"22","bibliographic_titles":[{"bibliographic_title":"近畿大学医学雑誌","bibliographic_titleLang":"ja"},{"bibliographic_title":"Medical Journal of Kinki University","bibliographic_titleLang":"en"}]}]},"item_2_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"近年，成熟破骨細胞が単離されるようになり，破骨細胞の骨吸収機構が明らかになりつつある．本研究はウサギ酒石酸抵抗性酸ホスファターゼ(TRAP)陽性細胞を精製し，骨吸収刺激因子，骨吸収抑制因子および合金のTRAP活性と線溶活性への影響を検討し，破骨細胞の骨吸収機構を解明することを目的とした．ウサギ全骨細胞より培養皿上に強固に吸着した細胞を単離した．この細胞は組織学的にTRAP染色および26C3モノクロナール抗体免疫染色陽性で破骨細胞の特性を有していた．TRAP陽性細胞を骨吸収刺激因子parathyroid hormone，prostaglandin E2 (PGE2)，1,25-dihydroxyvitamin D3，骨吸収抑制因子7-isopropoxyisoflavone (IP)，menatetrenone (Vit. K_2)，calcitonin(CT)，ethane-1-hydroxy-1, 1-diphosphonate (EHDP)および合金であるstainless，cobaltchrome-molybdnum，titanium-6aluminum-4vanadium合金で刺激し，培養上清液，細胞表面分画液，細胞溶解液をサンプルとした．フェニル燐酸基質法で測定したTRAP活性は培養上清液，細胞溶解液ではPGE_2，IP，Vit. K_2，CT，EHDPにより抑制された．TRAP陽性細胞に対するstainless，cobalt-chrome，titanium-aluminumの合金刺激はTRAP活性の増強をきたした．つぎに，同サンプルのurokinase-type plasminogen activator (u-PA活性)をエンザイモグラムで解析し，またL pyroglutamyl-glycyl-L-arginine-p-nitroanilide hydrochrolide (s-2444)合成基質を用いて，1本鎖u-PA (scu-PA)を測定した．骨吸収刺激因子，骨吸収抑制因子および合金刺激によりu-PA活性およびscu-PA量はTRAP活性と同様な変化を示した．今回の結果から，精製TRAP陽性細胞の線溶活性と骨吸収の関係が初めて示唆された．","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"本文データはCiNiiから複製したものである。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学医学会","subitem_publisher_language":"ja"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03858367","subitem_source_identifier_type":"PISSN"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kinki University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"板金, 寛昌","creatorNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"AN00063584-19970625-0007.pdf","format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://kindai.repo.nii.ac.jp/record/2001217/files/AN00063584-19970625-0007.pdf"},"version_id":"73cdd35f-ed77-4ae5-ba13-71cc64f986f6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"tartrate resistant acid phosphatase (TRAP)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"osteoclast","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"bone resorption","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"urokinase-type plasminogen activator (u-PA)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"single-chain form u-PA (scu-PA)","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ウサギtartrate resistant acid phosphatase陽性細胞の線溶活性に対する骨吸収刺激・抑制因子および合金の影響","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ウサギtartrate resistant acid phosphatase陽性細胞の線溶活性に対する骨吸収刺激・抑制因子および合金の影響","subitem_title_language":"ja"}]},"item_type_id":"2","owner":"42","path":["1714622708958"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-05-08"},"publish_date":"2024-05-08","publish_status":"0","recid":"2001217","relation_version_is_last":true,"title":["ウサギtartrate resistant acid phosphatase陽性細胞の線溶活性に対する骨吸収刺激・抑制因子および合金の影響"],"weko_creator_id":"42","weko_shared_id":-1},"updated":"2024-05-21T04:34:29.972205+00:00"}