{"created":"2024-04-23T07:56:42.067815+00:00","id":2001117,"links":{},"metadata":{"_buckets":{"deposit":"7115e14f-b27c-4f33-b713-68a015a87f45"},"_deposit":{"created_by":42,"id":"2001117","owners":[42],"pid":{"revision_id":0,"type":"depid","value":"2001117"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:02001117","sets":["14:923:1477:1713338090072"]},"author_link":[],"item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1998-06-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"36","bibliographicPageStart":"27","bibliographicVolumeNumber":"23","bibliographic_titles":[{"bibliographic_title":"近畿大学医学雑誌","bibliographic_titleLang":"ja"},{"bibliographic_title":"Medical Journal of Kinki University","bibliographic_titleLang":"en"}]}]},"item_2_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"SD-3212は新規に開発された抗不整脈剤で，Vaughan-Williams分類上I+III+IV群の作用を呈する抗不整脈剤と考えられているが，ナトリウムチャネル抑制の結合解離過程について十分明らかにされていない．そこで今回SD-3212のモルモット単離心室筋細胞におけるナトリウムチャネル抑制機序を明らかにすることを目的とした．雄性モルモット(250～300g)の心臓をLangendorff法にて灌流し，collagenaseを用いて単離心室筋細胞を作成し，SD-3212のモルモット単離心室筋細胞ナトリウム電流に対する抑制効果を全膜電位固定法を用いて測定した．SD-3212は-140mVの静止状態から-30mVへの脱分極により活性化されるナトリウム電流を用量依存性に減少させた．SD-3212はナトリウム電流の不活性化曲線を勾配因子に影響を与えずに過分極方向に偏位させた．2段パルス法によるナトリウム電流抑制発現過程の実験では，SD-3212は10msecの条件パルスではナトリウム電流を11.2%抑制し，500msecの条件パルスでは55.6%抑制した．これより求めた不活性化チャネルブロック/活性化チャネルブロック比は5.0であった．SD-3212は顕著な使用頻度依存性抑制を示した．不活性化されたナトリウム電流の回復過程は，速い成分の時定数(τf)がpH6.8 : 66.6±5.5msec, pH7.4 : 84.0±24.2msec, pH8.0 : 75.3±5.8msec, 遅い成分の時定数(τs)は，pH6.8 : 5954.1±1112.8msec, pH7.4 : 4563.5±638.3msec, pH8.0 : 2983.8±315.4msecと，遅い成分の時定数(τs)はアルカローシスによって有意に短縮した．以上よりSD-3212は，ナトリウムチャネルの不活性化状態に強い親和性を示し，ナトリウムチャネルからの解離速度に基づく分類からはintermediate kinetic drugに相当する薬剤であり，ナトリウムチャネル薬剤結合部位からは，疎水性経路を通って解離することが示唆された．","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"本文データはCiNiiから複製したものである。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学医学会","subitem_publisher_language":"ja"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03858367","subitem_source_identifier_type":"PISSN"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kinki University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"塚本, 哲也","creatorNameLang":"ja"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kinki University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"高井, 博之","creatorNameLang":"ja"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kinki University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"唐崎, 専也","creatorNameLang":"ja"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kinki University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"薮下, 博史","creatorNameLang":"ja"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kinki University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"石川, 欽司","creatorNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"AN00063584-19980625-0027.pdf","format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://kindai.repo.nii.ac.jp/record/2001117/files/AN00063584-19980625-0027.pdf"},"version_id":"18cfbb1e-f964-453d-9669-e8239ff2265d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"whole cell patch clamp technique","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"SD-3212","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"sodium current","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"pH","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"antiarrhythmic","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin 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