{"created":"2024-03-15T00:34:13.241407+00:00","id":2000946,"links":{},"metadata":{"_buckets":{"deposit":"8aeb6000-4702-457b-acf8-e4dc5c3f5cf2"},"_deposit":{"created_by":42,"id":"2000946","owners":[42],"pid":{"revision_id":0,"type":"depid","value":"2000946"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:02000946","sets":["14:923:1477:1709704794675"]},"author_link":[],"control_number":"2000946","item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999-06-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"186","bibliographicPageStart":"175","bibliographicVolumeNumber":"24","bibliographic_titles":[{"bibliographic_title":"近畿大学医学雑誌","bibliographic_titleLang":"ja"},{"bibliographic_title":"Medical Journal of Kinki University","bibliographic_titleLang":"en"}]}]},"item_2_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"ATLにおける各染色体領域のコピー数の変化を同定するためにcomparative genomic hybridizationを用いてATL由来細胞株8株とATL症例12例の末梢血液を解析した．細胞株において高頻度にコピー数増加を検出した領域は2p，7q，14qであり，最小共通領域はそれぞれ2p16-22，7q21-36，14q32であった．14q32と2p16-22にはそれぞれT-cell lymphoma/leukemia-1（TCL1）遺伝子とhuman T-cell leukemia virus enhancer factor（HTLF）遺伝子が存在する．ATL細胞株のFISHによる解析の結果TCL1とT-cell receptor α（TCR α）遺伝子の再構成はなく，14q32のコピー数増加があるにも拘らずTCL1の発現はなかった．さらに2pのコピー数増加を検出したATL細胞株にHTLF遺伝子の高発現はなかった．2p16-22と14q32の染色体領域にHTLFとTCL1以外のATLの不死化や白血病化に関与する遺伝子の存在が示唆された．double minute chromosome（dmin）は遺伝子増幅の場となる染色体異常として知られている．spectral karyotyping及びcomparative genomic hybridizationを用いてdminを持つ白血病細胞の増幅遺伝子の起源の同定を行った．急性骨髄性白血病（AML）細胞株KY821及びAML症例を対象とした．SKY法の結果からKY821のdminは8番染色体由来の染色体断片であることが明らかになった．CGH法により8q22-24のコピー数増加を検出し，c-myc遺伝子の増幅を明らかにした．AML症例のdminは11番染色体由来の断片であり，さらにpartial tandem duplicationを伴うmyeloid-lymphoid leukemia（MLL）の遺伝子増幅であることを明らかにした．","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"本文データはCiNiiから複製したものである。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学医学会","subitem_publisher_language":"ja"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03858367","subitem_source_identifier_type":"PISSN"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kinki University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"有山, 洋二","creatorNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"AN00063584-19990625-0175.pdf","format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://kindai.repo.nii.ac.jp/record/2000946/files/AN00063584-19990625-0175.pdf"},"version_id":"54e6aa5e-bbee-45cd-ba6e-0073ff03ee92"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"SKY","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"CGH","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ATL","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"dmin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"遺伝子増幅","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"MLL","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PTD","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"CGH法によるATLの解析ならびにSKY法，CGH法によるdminの染色体DNAの由来と増幅遺伝子の同定","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"CGH法によるATLの解析ならびにSKY法，CGH法によるdminの染色体DNAの由来と増幅遺伝子の同定","subitem_title_language":"ja"}]},"item_type_id":"2","owner":"42","path":["1709704794675"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-03-15"},"publish_date":"2024-03-15","publish_status":"0","recid":"2000946","relation_version_is_last":true,"title":["CGH法によるATLの解析ならびにSKY法，CGH法によるdminの染色体DNAの由来と増幅遺伝子の同定"],"weko_creator_id":"42","weko_shared_id":-1},"updated":"2024-04-24T07:05:00.088884+00:00"}