{"created":"2024-03-15T00:33:56.892749+00:00","id":2000941,"links":{},"metadata":{"_buckets":{"deposit":"13ac8b42-1030-4604-b945-7dc2ff5af6a7"},"_deposit":{"created_by":42,"id":"2000941","owners":[42],"pid":{"revision_id":0,"type":"depid","value":"2000941"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:02000941","sets":["14:923:1477:1709704794675"]},"author_link":[],"item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999-06-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"136","bibliographicPageStart":"129","bibliographicVolumeNumber":"24","bibliographic_titles":[{"bibliographic_title":"近畿大学医学雑誌","bibliographic_titleLang":"ja"},{"bibliographic_title":"Medical Journal of Kinki University","bibliographic_titleLang":"en"}]}]},"item_2_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"角膜創傷治癒におけるsecreted protein which is acidic and rich in cysteine (SPARC)の役割を検討するため，角膜実質細胞のα-smooth muscle actin (α-SMA)発現とSPARCの関係を検討した．家兎角膜に穿孔創を作成し，1週間後にSPARCおよびα-SMAに対する特異抗体を用いて免疫組織染色を行った．また，継代培養した家兎角膜実質細胞の培養液中にSPARCやtransforming growth factor-β (TGF-β)を添加し，細胞のα-SMA発現を免疫組織学的に検討した．さらに細胞のα-SMA発現量をwestern blotting法を用いて半定量した．正常家兎角膜では上皮および実質にSPARCの局在を認めなかった．一方，角膜損傷部の角膜上皮の基底細胞層，実質細胞およびその周囲にSPARCが発現し，同時に損傷部実質にα-SMAを発現した実質細胞が認められた．培養角膜実質細胞の培養液中にSPARCを添加することによりα-SMAを発現する細胞数がSPARCの濃度依存的に増加した．SPARC添加によって，角膜実質細胞が発現するα-SMA量が有意に増加していることがwestern blotting法で認められた．角膜実質細胞のα-SMA発現に対するSPARCの促進作用は，抗TGF-β抗体の添加によって抑制された．以上のことから角膜損傷部に出現するSPARCは実質細胞を活性化してmyofibroblastに変化させ，実質の創傷治癒に重要な役割を演じていることが示唆された．さらにこのSPARCの作用機序にTGF-βが関与していることが示唆された．","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"本文データはCiNiiから複製したものである。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学医学会","subitem_publisher_language":"ja"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03858367","subitem_source_identifier_type":"PISSN"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kinki University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"原, 英徳","creatorNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"AN00063584-19990625-0129.pdf","format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://kindai.repo.nii.ac.jp/record/2000941/files/AN00063584-19990625-0129.pdf"},"version_id":"d4046c8e-0f58-4395-ade3-7bf67d897850"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"corneal wound healing","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"keratocytes","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"secreted protein which is acidic and rich in cysteine (SPARC)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"α-smooth muscle actin (α-SMA)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"transforming growth factor-β (TGF-β)","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"角膜創傷治癒におけるSPARCの役割について","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"角膜創傷治癒におけるSPARCの役割について","subitem_title_language":"ja"}]},"item_type_id":"2","owner":"42","path":["1709704794675"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-03-15"},"publish_date":"2024-03-15","publish_status":"0","recid":"2000941","relation_version_is_last":true,"title":["角膜創傷治癒におけるSPARCの役割について"],"weko_creator_id":"42","weko_shared_id":-1},"updated":"2024-04-24T07:04:54.663798+00:00"}