{"created":"2024-03-15T00:33:20.217912+00:00","id":2000929,"links":{},"metadata":{"_buckets":{"deposit":"e999568c-c920-44f1-9c72-e6c0955f1a19"},"_deposit":{"created_by":42,"id":"2000929","owners":[42],"pid":{"revision_id":0,"type":"depid","value":"2000929"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:02000929","sets":["14:923:1477:1709704794675"]},"author_link":[],"item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999-06-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"32","bibliographicPageStart":"25","bibliographicVolumeNumber":"24","bibliographic_titles":[{"bibliographic_title":"近畿大学医学雑誌","bibliographic_titleLang":"ja"},{"bibliographic_title":"Medical Journal of Kinki University","bibliographic_titleLang":"en"}]}]},"item_2_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"キマーゼはアンジオテンシン変換酵素（ACE）と同様にアンジオテンシンII（AII）産生酵素であることが明らかになっている．本実験は心臓線維芽細胞におけるキマーゼとACE遺伝子の発現及びAIIに対する発現調節を調べ心臓の間質におけるAIIに対する自己調節機能（auto-regulation）を明らかにするために行った．3週令雄のシリアンハムスターの心臓を摘出しexplant法で心臓線維芽細胞を分離培養した．初代培養から第4継代までの細胞よりacid guanidinium thiocyanate-phenol-chloroform（AGPC）法を用いてtotal RNAを抽出しreverse transcription-polymerase chain reaction（RT-PCR）法でキマーゼとACEmessenger-RNA（m-RNA）の存在を確認した．キマーゼとACEm-RNAは第4継代の心臓線維芽細胞までほぼ同程度の発現が見られた．次に第3-4継代の細胞を用いてAII刺激を行い，competitive RT-PCR法でキマーゼとACEm-RNAの変化を見た．また1型アンジオテンシンII受容体拮抗薬（AT_1受容体拮抗薬）であるCV11974で前処置後AII刺激を行いキマーゼとACE m-RNAの変化を見た．AII刺激及びCV11974の前処置ではキマーゼm-RNAの発現量は変化が見られなかった．ACEm-RNAの発現量はAII刺激により濃度依存性に抑制され，CV11974の前処置により濃度依存性に抑制が改善された．この事よりキマーゼが心臓線維芽細胞で発現していることが明らかになりAII刺激によりACEは発現抑制を受けキマーゼは受けず，キマーゼとACEはAII刺激下のハムスター心臓線維芽細胞において発現調節が異なることが示唆された．","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"本文データはCiNiiから複製したものである。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学医学会","subitem_publisher_language":"ja"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03858367","subitem_source_identifier_type":"PISSN"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kinki University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"黒岡, 京浩","creatorNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"AN00063584-19990625-0025.pdf","format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://kindai.repo.nii.ac.jp/record/2000929/files/AN00063584-19990625-0025.pdf"},"version_id":"51e28998-8c46-4432-b30e-1b9ce51ed424"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"chymase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"fibroblasts","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"angiotensin-converting enzyme (ACE)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"competitive RT-PCR","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"angiotensin II","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ハムスター心臓線維芽細胞におけるアンジオテンシンII刺激に対するキマーゼとアンジオテンシン変換酵素（ACE）の発現について","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ハムスター心臓線維芽細胞におけるアンジオテンシンII刺激に対するキマーゼとアンジオテンシン変換酵素（ACE）の発現について","subitem_title_language":"ja"}]},"item_type_id":"2","owner":"42","path":["1709704794675"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-03-15"},"publish_date":"2024-03-15","publish_status":"0","recid":"2000929","relation_version_is_last":true,"title":["ハムスター心臓線維芽細胞におけるアンジオテンシンII刺激に対するキマーゼとアンジオテンシン変換酵素（ACE）の発現について"],"weko_creator_id":"42","weko_shared_id":-1},"updated":"2024-04-24T07:04:50.493596+00:00"}