{"created":"2023-11-08T06:05:15.991702+00:00","id":2000385,"links":{},"metadata":{"_buckets":{"deposit":"c9938a78-cb68-4d83-bbe7-bf90b442bfff"},"_deposit":{"created_by":42,"id":"2000385","owners":[42],"pid":{"revision_id":0,"type":"depid","value":"2000385"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:02000385","sets":["14:2667:1697004343623"]},"author_link":[],"item_1693381679919":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"report","resourceuri":"http://purl.org/coar/resource_type/c_93fc"}]},"item_30001_bibliographic_information17":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicNumberOfPages":"4","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2022)","bibliographic_titleLang":"ja"}]}]},"item_30001_creator2":{"attribute_name":"研究代表者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"34419","affiliationNameIdentifierScheme":"kakenhi"}],"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kindai University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"梅澤, 究","creatorNameLang":"ja"},{"creatorName":"Umezawa, Kiwamu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"70802544","nameIdentifierScheme":"e-Rad"}]}]},"item_30001_description8":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"褐色腐朽菌における結晶性セルロースの分解機構を明らかにすべく、申請者らが網羅的遺伝子発現解析から見いだした、木質基質により顕著に発現増加する3種類のタンパク質である、ファミリー9溶解性多糖モノオキシゲナーゼ（LPMO9）、ファミリー14 LPMO（LPMO14）、エクスパンシン様タンパク質の機能解析を行った。本研究では、これらの3種類のタンパク質を組換えタンパク質として発現させ、その糖質加水分解酵素との相乗作用および糖質結合能の調査を進めることで、その機能の解明につながる新たな知見を得ることができた。\n","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"To clarify the degradation mechanism of crystalline cellulose in brown rot fungi, the functions of three proteins, a family 9 lytic polysaccharide monooxygenase (LPMO9), a family 14 lytic polysaccharide monooxygenase (LPMO14) and an expansin-like protein, which significantly up-regulated by woody substrates were analysed. These three proteins were expressed as recombinant proteins and their synergistic effects with glycoside hydrolases and their carbohydrate-binding ability were investigated. These results provide new insights into their physiological functions in brown rot fungi.\n","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"研究分野：木材腐朽菌学","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_30001_file22":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"20K15568seika.pdf","format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://kindai.repo.nii.ac.jp/record/2000385/files/20K15568seika.pdf"},"version_id":"35f3fa19-f036-44da-9e2a-23a5b8a69986"}]},"item_30001_funding_reference15":{"attribute_name":"助成情報","attribute_value_mlt":[{"subitem_award_numbers":{"subitem_award_number":"20K15568","subitem_award_uri":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20K15568/"},"subitem_award_titles":[{"subitem_award_title":"褐色腐朽菌の結晶性セルロース分解におけるキー酵素の解明\n","subitem_award_title_language":"ja"},{"subitem_award_title":"Exploration of key enzymes for crystalline cellulose degradation in brown rot fungi","subitem_award_title_language":"en"}],"subitem_funder_names":[{"subitem_funder_name":"独立行政法人日本学術振興会","subitem_funder_name_language":"ja"},{"subitem_funder_name":"Japan Society for the Promotion of Science","subitem_funder_name_language":"en"}]}]},"item_30001_language10":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_30001_subject7":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"木材腐朽菌 　","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"褐色腐朽菌 　","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"木質バイオマス 　","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"バイオリファイナリー 　","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"糖質関連酵素","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_30001_title0":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"褐色腐朽菌の結晶性セルロース分解におけるキー酵素の解明","subitem_title_language":"ja"},{"subitem_title":"Exploration of key enzymes for crystalline cellulose degradation in brown rot fungi","subitem_title_language":"en"}]},"item_30001_version_type12":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_title":"褐色腐朽菌の結晶性セルロース分解におけるキー酵素の解明","item_type_id":"40001","owner":"42","path":["1697004343623"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-11-08"},"publish_date":"2023-11-08","publish_status":"0","recid":"2000385","relation_version_is_last":true,"title":["褐色腐朽菌の結晶性セルロース分解におけるキー酵素の解明"],"weko_creator_id":"42","weko_shared_id":-1},"updated":"2024-10-23T00:48:23.195189+00:00"}