@techreport{oai:kindai.repo.nii.ac.jp:02000378, author = {板倉, 修司 and Itakura, Shuji}, note = {有翅虫の特徴である複眼や翅の形成,幼形生殖虫の特徴である体表の着色やキチン合成への関与が予測される3種類のmiRNA(miR-7-5p,miR-8-3p,miR-12-5p)のinhibitorとmimicをイエシロアリとヤマトシロアリに注入した。miRNA inhibitor注入で,ヤマトシロアリではnostrin,ribosomal proteinなどのmRNA発現量が増加した。イエシロアリではCYP15A1,JHBP,エクジソン受容体などのmRNA発現量が増加した。miRNA mimic注入では,両シロアリで注入後5~7日経過後にJHAMTとCYP15A1のmRNA量の低下が認められた。, Three types of miRNAs (miR-7-5p,miR-8-3p,miR-12-5p) predicted to be involved in the formation of compound eyes or wings, which are characteristics of alates, in the body coloring, which are characteristics of replacement reproductive, and in the chitin synthesis, were selected as targets. Inhibitors or mimics of the miRNAs were injected into worker caste termites of Coptotermes formosanus and Reticulitermes speratus. Injection of miRNA inhibitors increased the expression of mRNAs such as nostrin and ribosomal protein in R. speratus. In C. formosanus, mRNA expression levels of CYP15A1, JHBP, and ecdysone receptor increased. Injection of miRNA mimic reduced the mRNA levels of JHAMT and CYP15A1 in both C. formosanus and R. speratus 5-7 days after injection., 研究分野:木材保存学}, title = {シロアリの形態変化におけるマイクロRNA機能解析と増殖個体の養殖飼料としての展開} }