{"created":"2023-11-08T01:38:50.400496+00:00","id":2000329,"links":{},"metadata":{"_buckets":{"deposit":"e8fd1360-7957-4812-a9a3-d2abc1ca2961"},"_deposit":{"created_by":42,"id":"2000329","owners":[42],"pid":{"revision_id":0,"type":"depid","value":"2000329"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:02000329","sets":["14:2667:1697004343623"]},"author_link":[],"item_1693381679919":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"report","resourceuri":"http://purl.org/coar/resource_type/c_93fc"}]},"item_30001_bibliographic_information17":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicNumberOfPages":"7","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2022)","bibliographic_titleLang":"ja"}]}]},"item_30001_creator2":{"attribute_name":"研究代表者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"34419","affiliationNameIdentifierScheme":"kakenhi"}],"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kindai University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"中澤, 直高","creatorNameLang":"ja"},{"creatorName":"Nakazawa, Naotaka","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"90800780","nameIdentifierScheme":"e-Rad"}]}]},"item_30001_description8":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"発生過程の小脳組織内を遊走する小脳顆粒細胞において、細胞核の物性に関わるLaminAタンパク質レベルを調べたところ、脳発生過程でそのレベルが変動することを突き止めた。LaminAを強制発現した小脳顆粒細胞では遊走効率が低下したことから、LaminAを介した細胞核弾性率の調節がニューロン遊走に関わることが示唆された。また、ニューロン核への機械的なストレスによって発生するDNA損傷については、狭小な空間を通過した際に53BPが一時的に凝集することを見出した。さらにin vitro実験系と薬剤処理による分子の撹乱実験により、上述のDNA損傷は非相同末端結合によって修復されることが示唆された。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"We found that LaminA protein levels fluctuated in migratory cerebellar granule cells during cerebellum development. In addition, overexpression of LMNA in cerebellar granule cells reduced migration efficiency. These suggest that LMNA-mediated regulation of nuclear stiffness is critical for neuronal migration. On the other hand, we found that 53BP transiently aggregated in the nucleus when the migratory neurons passed through confined space. Our experiments using in vitro system and inhibitor treatment revealed that DNA damage by mechanical stress in migratory neurons was repaired by non-homologous end joining.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"研究分野：神経科学、細胞生物学、発生生物学","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_30001_file22":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"18K14819seika.pdf","format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://kindai.repo.nii.ac.jp/record/2000329/files/18K14819seika.pdf"},"version_id":"260e89cd-1d00-42a1-b6f3-b37123e31b02"}]},"item_30001_funding_reference15":{"attribute_name":"助成情報","attribute_value_mlt":[{"subitem_award_numbers":{"subitem_award_number":"18K14819","subitem_award_uri":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18K14819/"},"subitem_award_titles":[{"subitem_award_title":"ニューロン遊走における細胞核弾性率の調節機構およびDNA損傷保護機構の解明","subitem_award_title_language":"ja"},{"subitem_award_title":"A study on the regulation of nuclear mechanics and DNA damage protection in neuronal migration","subitem_award_title_language":"en"}],"subitem_funder_names":[{"subitem_funder_name":"独立行政法人日本学術振興会","subitem_funder_name_language":"ja"},{"subitem_funder_name":"Japan Society for the Promotion of Science","subitem_funder_name_language":"en"}]}]},"item_30001_language10":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_30001_subject7":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ニューロン遊走","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞核","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"マイクロデバイス流路","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_30001_title0":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ニューロン遊走における細胞核弾性率の調節機構およびDNA損傷保護機構の解明","subitem_title_language":"ja"},{"subitem_title":"A study on the regulation of nuclear mechanics and DNA damage protection in neuronal migration","subitem_title_language":"en"}]},"item_30001_version_type12":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_title":"ニューロン遊走における細胞核弾性率の調節機構およびDNA損傷保護機構の解明","item_type_id":"40001","owner":"42","path":["1697004343623"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-11-08"},"publish_date":"2023-11-08","publish_status":"0","recid":"2000329","relation_version_is_last":true,"title":["ニューロン遊走における細胞核弾性率の調節機構およびDNA損傷保護機構の解明"],"weko_creator_id":"42","weko_shared_id":-1},"updated":"2024-10-23T00:48:27.547409+00:00"}