{"created":"2023-09-27T06:55:54.539007+00:00","id":2000121,"links":{},"metadata":{"_buckets":{"deposit":"e0542068-fd18-4053-82dd-44d348b10e96"},"_deposit":{"created_by":42,"id":"2000121","owners":[42],"pid":{"revision_id":0,"type":"depid","value":"2000121"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:02000121","sets":["14:923:1477:1695705542748"]},"author_link":[],"control_number":"2000121","item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2001-11-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"179","bibliographicPageStart":"171","bibliographicVolumeNumber":"26","bibliographic_titles":[{"bibliographic_title":"近畿大学医学雑誌","bibliographic_titleLang":"ja"},{"bibliographic_title":"Medical Journal of Kinki University","bibliographic_titleLang":"en"}]}]},"item_2_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"SPARC（Secreted protein which is acidic and rich in cysteine）は角膜実質創傷部に出現し，実質細胞の活性化に関与していると考えられている．培養家兎角膜実質細胞に対するSPARCの作用機序の詳細を知る目的で，SPARCのドメインII領域のペプチドKGHK，III領域のペプチド3.2，IV領域のペプチド4.2a，4.2a'，4.2bを作成した．それぞれのペプチドが角膜実質細胞のコラーゲンゲル収縮に及ぼす影響を検討した．ドメインIV領域由来のペプチド4.2a，4.2a'のみがコラーゲンゲル収縮抑制作用を示し，他のペプチドKGHK，3.2，4.2bはコラーゲンゲル収縮に影響を与えなかった．角膜実質細胞とI型コラーゲンとの接着に対するペプチド4.2aの影響を検討した．細胞培養用プラスチック製カバースリップをI型コラーゲンでコーティングした後，角膜実質細胞を播種した．ペプチド4.2aを添加し，経時的に蛍光画像解析装置を用いて細胞面積を計測し，走査型電子顕微鏡で細胞の形態を観察した．ペプチド4.2a添加は角膜実質細胞のコラーゲンへの接着・伸展を阻害し，反接着作用を示した．これらのことから，SPARCのドメインIV領域に実質細胞とコラーゲンとの接着・伸展を阻害する機能があることが明らかになった．","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"本文データはCiNiiから複製したものである。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学医学会","subitem_publisher_language":"ja"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03858367","subitem_source_identifier_type":"PISSN"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kinki Unversity","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"森下, 亮子","creatorNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"AN00063584-20011125-0171.pdf","format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://kindai.repo.nii.ac.jp/record/2000121/files/AN00063584-20011125-0171.pdf"},"version_id":"be9a99fb-e90b-49bc-bccd-3bbd76af8213"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"角膜実質","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"角膜上皮","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"角膜創傷治癒","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"SPARCペプチド","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"I型コラーゲン","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞接着","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"角膜創傷治癒に及ぼすSPARC由来ペプチドの影響","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"角膜創傷治癒に及ぼすSPARC由来ペプチドの影響","subitem_title_language":"ja"}]},"item_type_id":"2","owner":"42","path":["1695705542748"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-09-27"},"publish_date":"2023-09-27","publish_status":"0","recid":"2000121","relation_version_is_last":true,"title":["角膜創傷治癒に及ぼすSPARC由来ペプチドの影響"],"weko_creator_id":"42","weko_shared_id":-1},"updated":"2023-10-12T00:38:57.158406+00:00"}