{"created":"2023-06-20T16:48:04.370458+00:00","id":19876,"links":{},"metadata":{"_buckets":{"deposit":"d2257094-62e6-4280-866c-d394d8734271"},"_deposit":{"created_by":3,"id":"19876","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"19876"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00019876","sets":["14:923:1809:4478"]},"author_link":["33779","33780","33781","33782","33783","33784","33785"],"control_number":"19876","item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-11-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"29","bibliographicPageEnd":"5","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"かやのもり：近畿大学産業理工学部研究報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"Reports of Faculty of Humanity-Oriented Science and Engineering, Kindai University","bibliographic_titleLang":"en"}]}]},"item_2_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"KRAS mutation is positive in 45% of colon cancer patients, 35% of lung cancer patients, 95% of pancreas cancer patients, and 15% of melanoma patients and the patients do not benefit from anti-epidermal growth factor receptor (EGFR) chemotherapy and antibody therapy to have poor prognosis for survival. KRAS mutations in codons 12 and 13 were present approximately in 40% of colorectal cancers. It is highly challenging to target mutant KRAS gene by synthetic small nucleic acids and can be a breakthrough for undruggable cancers.In the present study, we investigated silencing efficiencies of mutant KRAS(G12D) gene by gapmer antisense oligonucleotides (ASO) using HeLa with wild type KRAS and PK-45H with G12D mutation in both alleles. ASOs targeting mutant KRAS (G12D) with 11nt and 9nt phosphorothioate gap silenced KRAS(G12D) expression in 81% and 73% efficiency in PK-45H, respectively but affected very little on the wild type KRAS expression in HeLa.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_2_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15100/0000019876","subitem_identifier_reg_type":"JaLC"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学産業理工学部"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13495801","subitem_source_identifier_type":"ISSN"}]},"item_2_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学産業理工学部生物環境化学科; 博士研究員"},{"subitem_text_value":"近畿大学産業理工学部生物環境化学科"},{"subitem_text_value":"近畿大学産業理工学部生物環境化学科; 教授"},{"subitem_text_value":"マンチェスター大学; 教授"},{"subitem_text_value":"サルフォード大学; 教授"},{"subitem_text_value":"サルフォード大学; 教授"},{"subitem_text_value":"近畿大学産業理工学部生物環境化学科; 教授"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kindai University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"塩浜, 康雄","creatorNameLang":"ja"},{"creatorName":"シハマ, ヤスオ","creatorNameLang":"ja-Kana"},{"creatorName":"Shihama,Yasuo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kindai University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"藤田, 崇史","creatorNameLang":"ja"},{"creatorName":"フジタ, タカシ","creatorNameLang":"ja-Kana"},{"creatorName":"Fujita,Takashi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kindai University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"神武, 洋二郎","creatorNameLang":"ja"},{"creatorName":"コウタケ, ヨウジロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Kotake,Yojiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Demonacos, Constantinos","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Krstic-Demonacos, Marija","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Di Leva, Gianpiero","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kindai University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"藤井, 政幸","creatorNameLang":"ja"},{"creatorName":"フジイ, マサユキ","creatorNameLang":"ja-Kana"},{"creatorName":"Fujii,Masayuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","displaytype":"detail","filename":"AA12013209-20181130-0001.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"url":"https://kindai.repo.nii.ac.jp/record/19876/files/AA12013209-20181130-0001.pdf"},"version_id":"f940399a-6970-4360-982d-c5349825e151"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Mutant KRAS Positive Cancer","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Nucleic Acid Therapeutics","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Gapmer Antisense Oligonucleotides","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"〈論文・報告〉Gapmerアンチセンス核酸による変異癌遺伝子KRAS（G12D）の選択的発現制御","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"〈論文・報告〉Gapmerアンチセンス核酸による変異癌遺伝子KRAS（G12D）の選択的発現制御","subitem_title_language":"ja"},{"subitem_title":"〈PAPERS and REPORTS〉Selective Regulation of Mutant Oncogene KRAS (G12D) by Gapmer Antisense Nucleic Acids","subitem_title_language":"en"}]},"item_type_id":"2","owner":"3","path":["4478"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-01-28"},"publish_date":"2019-01-28","publish_status":"0","recid":"19876","relation_version_is_last":true,"title":["〈論文・報告〉Gapmerアンチセンス核酸による変異癌遺伝子KRAS（G12D）の選択的発現制御"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-09-14T08:00:04.515278+00:00"}